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首页> 外文期刊>Journal of Experimental Marine Biology and Ecology >Chromosomal mapping of rDNAs, core histone genes and telomeric sequences in Perumytilus purpuratus (Bivalvia: Mytilidae)
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Chromosomal mapping of rDNAs, core histone genes and telomeric sequences in Perumytilus purpuratus (Bivalvia: Mytilidae)

机译：紫癣菌（双壳菌：Mytilidae）中rDNA，核心组蛋白基因和端粒序列的染色体作图

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摘要

The chromosomes of the mussel Perumytilus purpuratus were analyzed by means of 4,6-diamidino-2-phenylindole (DAPI)/propidium iodide (PI) and chromomycin A3 (CMA)/DAPI fluorescence staining and fluorescent in situ hybridization (FISH) with major rDNA, 5S rDNA, core histone gene and telomeric probes. The diploid chromosome number in this species is 32. The karyotype is composed by two metacentric, five submetacentric, one subtelo/submetacentric and eight subtelocentric chromosome pairs. The nudeolar organizing regions (NORs) detected by FISH using major rDNA probes were terminally located on the long arms of two pairs of chromosomes. NORs were associated with bright CMA fluorescence and dull DAPI fluorescence but not all the NORs showed bright CMA fluorescence in a given cell; intra- and inter-individual variability was found in this character. FISH detected a similar variability on the number of major rDNA signals. This species shows three 5S rDNA clusters on two chromosome pairs. Two of the clusters are terminal and intercalary located on one of the two NOR-bearing chromosome pairs; the third cluster is on the long arm of another chromosome pair. Core histone genes are clustered on a single locus near the centromere on the long arm of a different chromosome pair. This locus is not condensed in prophase I cells therefore suggesting a certain degree of histone gene expression during meiosis. As expected, P. purpuratus shows telomeric signals at both ends of every single chromosome but interstitial telomeric signals were also detected at centromeric positions on two chromosome pairs.
机译：主要通过4,6-二mid基-2-苯基吲哚（DAPI）/碘化丙啶（PI）和嗜铬霉素A3（CMA）/ DAPI荧光染色和原位荧光杂交（FISH）分析贻贝紫癜的染色体rDNA，5S rDNA，核心组蛋白基因和端粒探针。该物种的二倍体染色体数为32。核型由两个亚中心，五个亚亚中心，一个亚亚/亚亚中心和八对亚中心染色体组成。 FISH使用主要的rDNA探针检测到的核仁组织区（NORs）末端位于两对染色体的长臂上。 NOR与明亮的CMA荧光和暗淡的DAPI荧光有关，但并非所有NOR在给定的细胞中均显示明亮的CMA荧光。在这个角色中发现了个体内部和个体之间的变异性。 FISH在主要rDNA信号的数量上检测到相似的变异性。该物种在两个染色体对上显示三个5S rDNA簇。其中两个簇位于两个带有NOR的染色体对之一上，位于末端和中间。第三簇位于另一对染色体的长臂上。核心组蛋白基因聚集在不同染色体对长臂着丝粒附近的单个基因座上。该基因座在前期I细胞中不浓缩，因此提示减数分裂过程中一定程度的组蛋白基因表达。不出所料，紫癜疟原虫在每条染色体的两端均显示端粒信号，但在两个染色体对的着丝粒位置也检测到间质端粒信号。

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