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Application of high density steam flash-explosion in protein extraction of soybean meal

机译：高密度蒸汽闪爆在大豆粉蛋白质提取中的应用

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摘要

The high density steam flash-explosion (HDSFE) was used to extract protein from soybean meal. Soybean meal samples were treated at 1.3 MPa and 1.8 MPa for 60 s, 120 s and 180 s, respectively. After HDSFE treatment at 1.8 MPa for 180 s, the extraction yield of protein was increased from 50.50% to 65.66% compared with untreated soybean meal. The emulsification properties and fat-binding capacity of soy protein isolate (SPI) extracted from soybean meal treated by HDSFE were all improved compared with SP1 extracted from untreated soybean meal and white flakes. Molecular weight distribution analysis of SPI showed that after HDSFE treatment the peak with molecular weight about 504 kDa and 43.3 kDa disappeared and the peak with molecular weight about 669 kDa increased indicating protein aggregation. Gel electrophoresis showed that high molecular weight aggregates of protein have been formed by covalent bond.
机译：高密度蒸汽闪蒸（HDSFE）用于从豆粕中提取蛋白质。大豆粉样品分别在1.3 MPa和1.8 MPa下处理60 s，120 s和180 s。 HDSFE在1.8 MPa下处理180 s后，与未经处理的豆粕相比，蛋白质的提取率从50.50％增加到65.66％。与未经处理的豆粕和白色薄片提取的SP1相比，经HDSFE处理的豆粕提取的大豆分离蛋白（SPI）的乳化性能和脂肪结合能力均得到改善。 SPI的分子量分布分析表明，HDSFE处理后，分子量约为504 kDa和43.3 kDa的峰消失了，分子量约为669 kDa的峰增加了，表明蛋白质聚集。凝胶电泳显示，高分子量的蛋白质聚集体已经通过共价键形成。

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  • 来源
    《Journal of food engineering》 |2013年第2期|430-435|共6页
  • 作者

    Yanpeng Zhang; Ruijin Yang; Wei Zhao; Xiao Hua; Wenbin Zhang;

  • 作者单位

    State Key Laboratory of Food Science and Technology, Jiangnan University, 1800, Lihu Ave., Wuxi, Jiangsu 214122, China;

    School of Food Science and Technology, Jiangnan University, 1800, Lihu Ave., Wuxi, Jiangsu 214122, China;

    School of Food Science and Technology, Jiangnan University, 1800, Lihu Ave., Wuxi, Jiangsu 214122, China;

    State Key Laboratory of Food Science and Technology, Jiangnan University, 1800, Lihu Ave., Wuxi, Jiangsu 214122, China;

    State Key Laboratory of Food Science and Technology, Jiangnan University, 1800, Lihu Ave., Wuxi, Jiangsu 214122, China;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    high density steam flash-explosion; soybean meal; soy protein isolate; functional properties;

    机译：高密度蒸汽闪爆;豆粕大豆分离蛋白;功能特性;

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