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首页> 外文期刊>Journal of Food Processing and Preservation >Optimization of ultrasonic–microwave synergistic extraction of flavonoids from sweet potato leaves by response surface methodology
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Optimization of ultrasonic–microwave synergistic extraction of flavonoids from sweet potato leaves by response surface methodology

机译：响应面法优化超声波-微波协同提取红薯叶中黄酮类成分

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摘要

Ultrasonic-microwave synergistic extraction was used to extract flavonoids from sweet potato leaves (SPL) by response surface methodology. The optimal conditions for flavonoids extraction were 1:40 (g/ml) of solid-liquid ratio, 57 degrees C of extraction temperature, 76s of extraction time, and 72% (v/v) ethanol for 2 times, the highest extraction efficiency was 91.653.37%. After purification, the flavonoids purity reached to 76.10 +/- 3.11 (%, DW). The result of high-performance liquid chromatography revealed 11 compounds including astragalin (473.8 +/- 7.3mg/g, DW), quercetrin (86.5 +/- 0.7mg/g, DW), 4,5-chlorogenic acid (76.4 +/- 0.5mg/g, DW), isoquercitrin (62.4 +/- 0.4mg/g, DW), tiliroside (18.8 +/- 0.3mg/g, DW), quercetin (12.5 +/- 0.2mg/g, DW), 3,4,5-chlorogenic acid (6.5 +/- 0.2mg/g, DW), caffeic acid (6.1 +/- 0.2mg/g, DW), kaempferol (6.0 +/- 0.2mg/g, DW), myricetrin (5.9 +/- 0.1mg/g, DW), and rhamnetin (4.3 +/- 0.1mg/g, DW) in sweet potato leaf flavonoids, which possessed good antioxidant activity compared to soy isoflavones, ginkgo biloba extract, and propolis flavone. The IC50 value of sweet potato leaf flavonoids was 13.26 +/- 0.09g/ml in ferric reducing antioxidant power and 5.41 +/- 0.21 in 2, 2-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) scavenging capacity, respectively.Practical applicationsChina is the leading country of sweet potato production in the world, but sweet potato leaves (SPL) have been neglected except for a partial use as livestock feed in most parts of China, and detailed reports on the effective components in the leaves are scarce. Ultrasonic-microwave synergistic extraction is an efficient way to select flavonoids from SPL which has a potential to be extended in natural flavonoids industry.
机译：超声波-微波协同提取用于响应面法从甘薯叶中提取黄酮类化合物。黄酮类化合物的最佳提取条件为固液比为1:40（g / ml），提取温度为57℃，提取时间为76s，乙醇（v / v）为2倍（v / v）的2次，提取效率最高。为91.653.37％。纯化后，类黄酮的纯度达到76.10 +/- 3.11（％，DW）。高效液相色谱法的结果显示11种化合物，包括黄芪素（473.8 +/- 7.3mg / g，DW），槲皮素（86.5 +/- 0.7mg / g，DW），4,5-绿原酸（76.4 + / -0.5mg / g，DW），异槲皮苷（62.4 +/- 0.4mg / g，DW），tiliroside（18.8 +/- 0.3mg / g，DW），槲皮素（12.5 +/- 0.2mg / g，DW） ，3,4,5-绿原酸（6.5 +/- 0.2mg / g，DW），咖啡酸（6.1 +/- 0.2mg / g，DW），山奈酚（6.0 +/- 0.2mg / g，DW）甘薯叶类黄酮中的洋甘菊，杨梅素（5.9 +/- 0.1mg / g，DW）和鼠李素（4.3 +/- 0.1mg / g，DW），与大豆异黄酮，银杏叶提取物和蜂胶黄酮。甘薯叶黄酮的IC50值在三氧化二铁还原抗氧化剂能力中为13.26 +/- 0.09g / ml，在2，2-叠氮基双（3-乙基苯并噻唑啉-6-磺酸）清除能力中的IC50值分别为5.41 +/- 0.21实际应用中国是世界上甘薯生产的主要国家，但除中国大部分地区仅将其用作家畜饲料外，甘薯叶（SPL）一直被忽略，有关叶中有效成分的详细报道是稀缺。超声波-微波协同萃取是从SPL中选择黄酮类化合物的有效方法，它有可能在天然黄酮类化合物工业中得到推广。

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    Liu Jiang; Mu Taihua; Sun Hongnan; Fauconnier Marie Laure;

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    Chinese Acad Agr Sci, Minist Agr, Key Lab Agroprod Proc, 2 Yuanmingyuan West Rd,POB 5109, Beijing 100193, Peoples R China|Chinese Acad Agr Sci, Inst Food Sci & Technol, 2 Yuanmingyuan West Rd,POB 5109, Beijing 100193, Peoples R China|Univ Liege, Lab Gen & Organ Chem, Gembloux Agrobio Tech, Gembloux, Belgium;

    Chinese Acad Agr Sci, Minist Agr, Key Lab Agroprod Proc, 2 Yuanmingyuan West Rd,POB 5109, Beijing 100193, Peoples R China|Chinese Acad Agr Sci, Inst Food Sci & Technol, 2 Yuanmingyuan West Rd,POB 5109, Beijing 100193, Peoples R China;

    Chinese Acad Agr Sci, Minist Agr, Key Lab Agroprod Proc, 2 Yuanmingyuan West Rd,POB 5109, Beijing 100193, Peoples R China|Chinese Acad Agr Sci, Inst Food Sci & Technol, 2 Yuanmingyuan West Rd,POB 5109, Beijing 100193, Peoples R China;

    Univ Liege, Lab Gen & Organ Chem, Gembloux Agrobio Tech, Gembloux, Belgium;

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