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首页> 外文期刊>Journal of Food Processing and Preservation >Fermentation strategies for improving the production of bacteriocin-like inhibitory substances by Lactobacillus brevis C23 with nutrient supplementation, pH, and temperature variations
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Fermentation strategies for improving the production of bacteriocin-like inhibitory substances by Lactobacillus brevis C23 with nutrient supplementation, pH, and temperature variations

机译：通过Lactobacillus Brevis C23通过营养补充，pH和温度变化来改善乳杆菌样抑制物质生产的发酵策略

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摘要

In this study, fermentation strategies were carried out for improving the productionof bacteriocin-likeinhibitory substances by Lactobacillus brevis C23 with nutrient supplementation,pH, and temperature variations, as well as fed-batchcultivation. TheBLIS activity was highest (91.52%) in the MRS medium supplemented with 1.5% (w/v)lactose and 1% (w/v) meat extract. pH 5 augmented both the cell biomass and BLISactivity, but incubation temperature only affected growth at 37°C. Fed-batchcultivationstrategy that utilized meat extract feeding would positively affect cell growth,whereas BLIS activity was enhanced by lactose feeding. The highest BLIS activityfrom L. brevis C23 was obtained at the 24 hr mark by feeding the culture medium withlactose (77.73%). The BLIS production of L. brevis C23 fed with meat extract followeda similar trend to lactose (74.20%). Increased BLIS activity in the final solution wouldreduce the downstream step such as concentrating the purified product.
机译：在这项研究中，进行了发酵策略来改善生产细菌素样乳酸杆菌C23的抑制物质具有营养补充，pH和温度变化，以及FED批次栽培。这在补充1.5％（W / V）的MRS培养基中，BLIS活性最高（91.52％）乳糖和1％（w / v）肉提取物。 pH 5增强了细胞生物量和蓝色活性，但孵育温度仅在37°C下生长。 FED批量栽培利用肉类提取物喂养的策略会对细胞生长产生积极影响细胞生长乳糖喂养增强了Blis活性。最高的BLIS活动通过喂养培养基，在24小时标记中获得Brevis C23。通过喂养培养基乳糖（77.73％）。 L. Brevis C23的Blis生产喂养肉提取物乳糖的类似趋势（74.20％）。在最终解决方案中增加了BLIS活动减少下游步骤，例如浓缩纯化产物。

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  • 来源
    《Journal of Food Processing and Preservation》 |2021年第11期|e15914.1-e15914.13|共13页
  • 作者

    Dharni Kuhan Sreedharan; Sahar Abbasiliasi; Mohd Shamzi Mohamed; Zhang Jin Ng; Arbakariya Bin Ariff; Chee Keong Lee; Joo Shun Tan;

  • 作者单位

    School of Industrial Technology UniversitiSains Malaysia Gelugor Malaysia;

    Halal Products Research Institute Universiti Putra Malaysia Serdang Malaysia;

    Department of Bioprocess Technology Faculty of Biotechnology and BiomolecularSciences Universiti Putra Malaysia Serdang Malaysia Bioprocessing and BiomanufacturingResearch Complex Faculty of Biotechnologyand Biomolecular Sciences Universiti PutraMalaysia Serdang Malaysia;

    School of Industrial Technology UniversitiSains Malaysia Gelugor Malaysia;

    Department of Bioprocess Technology Faculty of Biotechnology and BiomolecularSciences Universiti Putra Malaysia Serdang Malaysia Bioprocessing and BiomanufacturingResearch Complex Faculty of Biotechnologyand Biomolecular Sciences Universiti PutraMalaysia Serdang Malaysia;

    School of Industrial Technology UniversitiSains Malaysia Gelugor Malaysia;

    School of Industrial Technology UniversitiSains Malaysia Gelugor Malaysia;

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  • 正文语种 eng
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