• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of food protection >Rapid separation and counting of viable microbial cells in food by nonculture method with bioplorer, a focusing-free microscopic apparatus with a novel cell separation unit
【24h】

Rapid separation and counting of viable microbial cells in food by nonculture method with bioplorer, a focusing-free microscopic apparatus with a novel cell separation unit

机译：通过非培养方法使用生物螺帽（一种具有新型细胞分离单元的免聚焦显微镜设备），通过非培养方法快速分离和计数食品中的活微生物细胞

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A nonculture method utilizing a novel apparatus, the bioplorer, was developed. The bioplorer is composed of an efficient cell separation unit, a focusing-free microscopic device, and an image analysis program. A meat or vegetable suspension is poured into the cell separation funnel, and insoluble matter in the sample suspension is trapped by prefilters. Microbial cells passing through the two prefilters are then trapped by the membrane filter (pore size, 0.4 microm). Trapped cells are double-stained with 4',6'-diamidino-2-phenylindole and propidium iodide, and the membrane filter is removed and set on the focusing-free microscope. A fluorescent image is then recorded. Total numbers of viable and dead cells on the membrane filter can thus be determined automatically. One assay can be performed within 10 min, which is much faster than the culture method. The results obtained with both the nonculture method and the culture method for meat and vegetable samples were highly correlated (r = 0.953 to 0.998). This method is feasible for the practical purpose of food safety control.
机译：开发了一种利用新型设备bioplorer的非培养方法。该生物聚结器由高效的细胞分离装置，无聚焦显微镜设备和图像分析程序组成。将肉类或蔬菜悬浮液倒入细胞分离漏斗中，并将样品悬浮液中的不溶物通过预过滤器捕集。然后，通过两个预滤器的微生物细胞被膜滤器捕获（孔径为0.4微米）。用4'，6'-二mid基-2-苯基吲哚和碘化丙啶对捕获的细胞进行双重染色，然后取下滤膜并放在无聚焦显微镜下。然后记录荧光图像。因此可以自动确定膜滤器上活细胞和死细胞的总数。一种分析可以在10分钟内完成，这比培养方法要快得多。用非培养法和肉类和蔬菜样品的培养方法获得的结果高度相关（r = 0.953至0.998）。该方法对于食品安全控制的实际目的是可行的。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号