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首页> 外文期刊>Journal of Food Science >Molecular Cloning and Characterizations of Xylanase Inhibitor Protein from Wheat (Triticum Aestivum)
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Molecular Cloning and Characterizations of Xylanase Inhibitor Protein from Wheat (Triticum Aestivum)

机译：小麦木糖木聚糖酶抑制剂蛋白的分子克隆与表征

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摘要

Xylanase inhibitor proteins (XIPs) were regarded to inhibit the activity of xylanases during baking and gluten-starch separation processes. To avoid the inhibition to xylanases, it is necessary to define the conditions under which the inhibition takes place. In this study, we cloned the XIP gene from 2 different variety of Triticum aestivum, that is, Zhengmai 9023 and Zhengmai 366, and investigated the properties of XIP protein expressed by Pichia pastoris. The results showed that the 2 XIP genes (xip-9023 and xip-366) were highly homologous with only 3 nucleotide differences. XIP-9023 showed the optimal inhibition pH and temperature were 7 ℃ and 40 ℃, respectively. Inhibition of xylanase by XIP-9023 reached the maximum in 40 min. At 50% inhibition of xylanase, the molar ratio of inhibitor: xylanase was 26:1. XIP-9023 was active to various fungal xylanases tested as well as to a bacterial xylanase produced by Paenibacillus sp. isolated from cow rumen.
机译：木聚糖酶抑制剂蛋白（XIP）被认为在烘烤和面筋-淀粉分离过程中抑制木聚糖酶的活性。为了避免对木聚糖酶的抑制，有必要定义抑制发生的条件。在这项研究中，我们从两个不同的小麦品种郑麦9023和郑麦366中克隆了XIP基因，并研究了毕赤酵母表达的XIP蛋白的特性。结果表明，两个XIP基因（xip-9023和xip-366）高度同源，仅有3个核苷酸差异。 XIP-9023的最佳抑菌pH和温度分别为7℃和40℃。 XIP-9023对木聚糖酶的抑制作用在40分钟内达到最大值。在木聚糖酶的抑制率为50％时，抑制剂：木聚糖酶的摩尔比为26∶1。 XIP-9023对测试的各种真菌木聚糖酶以及Paenibacillus sp。产生的细菌木聚糖酶均具有活性。从牛瘤胃分离。

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  • 来源
    《Journal of Food Science》 |2017年第9期|1582-1587|共6页
  • 作者

    Xinyu Liu; Yakun Zhang; Zhaohui Wei; Hongge Chen; Xincheng Jia;

  • 作者单位

    Key Laboratory of Enzyme Engineering of Agricultural Microbiology, Ministry of Agriculture, College of Life Sciences, Henan Agricultural Univ., Zhengzhou, Henan Province, 450002, PR. China.;

    Key Laboratory of Enzyme Engineering of Agricultural Microbiology, Ministry of Agriculture, College of Life Sciences, Henan Agricultural Univ., Zhengzhou, Henan Province, 450002, PR. China.;

    Key Laboratory of Enzyme Engineering of Agricultural Microbiology, Ministry of Agriculture, College of Life Sciences, Henan Agricultural Univ., Zhengzhou, Henan Province, 450002, PR. China.;

    Key Laboratory of Enzyme Engineering of Agricultural Microbiology, Ministry of Agriculture, College of Life Sciences, Henan Agricultural Univ., Zhengzhou, Henan Province, 450002, PR. China.;

    Key Laboratory of Enzyme Engineering of Agricultural Microbiology, Ministry of Agriculture, College of Life Sciences, Henan Agricultural Univ., Zhengzhou, Henan Province, 450002, PR. China.;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    inhibition characterization; molecular cloning; wheat; xylanase inhibitor protein;

    机译：抑制特性分子克隆小麦;木聚糖酶抑制剂蛋白;

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