...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Food Science >Detection of Viable Cronobacter spp. (Enterobacter sakazakii) by One-Step RT-PCR in Dry Aquatic Product
【24h】

Detection of Viable Cronobacter spp. (Enterobacter sakazakii) by One-Step RT-PCR in Dry Aquatic Product

机译：检测活的克罗诺杆菌属。干水产品中一步法RT-PCR检测（阪崎肠杆菌）

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Cronobacter are opportunistic food-borne pathogens associated with meningitis, sepsis, and necrotizing en-terocolitis. Little attempt has focused on detection of viable cell of Cronobacter spp. in dry aquatic products, which were frequently used for raw materials of infant foods due to high nutrition. In this paper, one-step reverse transcription polymerase chain reaction (RT-PCR) was developed for detection of viable Cronobacter spp. in dry aquatic products. Specificity test indicated that clearly expected amplicon in size 469bp was amplified from RNA of Cronobacter, but not from RNA of negative controls and DNA of Cronobacter strains. The sensitivity was 10~4 CFU/mL of Cronobacter strain in artificially fish meal samples and 10~1 CFU/mL of Cronobacter after 10-h enrichment. In a total of 81 dry aquatic products, 9.8%, 8.6%, and 9.8% of samples were found to be positive for Cronobacter by one-step RT-PCR, U.S. Food and Drug Administration method, and Druggan-Forsythe-Iversen medium, respectively. The results clearly indicated that one-step RT-PCR could avoid the interference of residual DNA of Cronobacter in food samples and be used to specifically detect viable Cronobacter spp. for large-scale monitoring of food samples.
机译：克罗诺杆菌是与脑膜炎，败血症和坏死性小肠结肠炎相关的机会性食源性病原体。很少尝试集中在检测克氏杆菌属的活细胞。在干燥的水产品中，由于营养丰富，常被用作婴儿食品的原料。本文开发了一步法逆转录聚合酶链反应（RT-PCR），用于检测活的克罗诺杆菌属。在干燥的水产品中。特异性测试表明，明显预期的扩增子大小为469bp，是从克罗诺杆菌的RNA扩增而来的，而不是阴性对照的RNA和克罗诺杆菌菌株的DNA。人工鱼粉样品中的克氏杆菌菌株的敏感性为10〜4 CFU / mL，富集10 h后的敏感性为10〜1 CFU / mL的克罗细菌。通过一步式RT-PCR，美国食品药品监督管理局方法和Druggan-Forsythe-Iversen培养基，在总共81种干水产品中，发现9.8％，8.6％和9.8％的样品对克罗诺杆菌呈阳性，分别。结果清楚地表明，一步法RT-PCR可以避免食品中克氏杆菌残留DNA的干扰，可用于特异性检测活的克氏杆菌属。用于食品样本的大规模监控。

著录项

  • 来源
    《Journal of Food Science》 |2012年第12期|M616-M619|共4页
  • 作者

    Yingwang Ye; Qingping Wu; jumei Zhang; He Jiang; Wang Hu;

  • 作者单位

    School of Biotechnology and Food Engineering, Hefei Univ. of Technology, Hefei, 230009,China,Guangdong Provincial Key Laboratory of Microbiology Culture Collection and Application, Guangdong Inst. of Microbiology, Guangzhou, 510010, China;

    Guangdong Provincial Key Laboratory of Microbiology Culture Collection and Application, Guangdong Inst. of Microbiology, Guangzhou, 510010, China;

    Guangdong Provincial Key Laboratory of Microbiology Culture Collection and Application, Guangdong Inst. of Microbiology, Guangzhou, 510010, China;

    Inst. of Fishery, Anhui Academy of Agricultural Sciences, Hefei, 230039, China;

    Inst. of Fishery, Anhui Academy of Agricultural Sciences, Hefei, 230039, China;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    cronobacter; DFI medium; FDA method; one-step RT-PCR;

    机译：克罗诺杆菌DFI介质;FDA方法;一步式RT-PCR;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号