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首页> 外文期刊>Journal of Hazardous Materials >Molecular mechanism of catalase activity change under sodium dodecyl sulfate-induced oxidative stress in the mouse primary hepatocytes
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Molecular mechanism of catalase activity change under sodium dodecyl sulfate-induced oxidative stress in the mouse primary hepatocytes

机译：十二烷基硫酸钠诱导小鼠原代肝细胞氧化应激下过氧化氢酶活性变化的分子机制

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摘要

Sodium dodecyl sulfate (SDS) contributes to adverse effects of organisms probably because of its ability to induce oxidative stress via changing the activity of antioxidant enzyme catalase (CAT). But the underlying molecular mechanisms still remain unclear. This study characterized the harmful effects of SDS-induced oxidative stress on the mouse primary hepatocytes as well as the structure and function of CAT molecule and investigated the underlying molecular mechanism. After 12 h SDS (0.1 mu M to 0.2 mM) exposure, no significant change was observed in CAT activity of the hepatocytes. After 0.5 and 0.8 mM SDS exposure, the state of oxidative stress stimulated CAT production in the hepatocytes. The inhibition of CAT activity induced by directly interacting with SDS was unable to catch the synthesis of CAT and therefore resulted in the increased activity and elevated ROS level. Further molecular experiments showed that SDS prefers to bind to the interface with no direct effect on the active site and the structure of heme groups of CAT molecule. When the sites in the interface is saturated, SDS interacts with VAL 73, HIS 74, ASN 147 and PHE 152, the key residues of the enzyme activity, and leads to the decrease of CAT activity. (C) 2015 Elsevier B.V. All rights reserved.
机译：十二烷基硫酸钠（SDS）可能对生物产生不利影响，可能是因为它能够通过改变抗氧化酶过氧化氢酶（CAT）的活性来诱导氧化应激。但是潜在的分子机制仍然不清楚。这项研究表征了SDS诱导的氧化应激对小鼠原代肝细胞的有害作用以及CAT分子的结构和功能，并研究了其潜在的分子机制。 SDS（​​0.1μM至0.2 mM）暴露12 h后，未观察到肝细胞CAT活性的显着变化。在0.5和0.8 mM SDS暴露后，氧化应激状态刺激了肝细胞中CAT的产生。通过直接与SDS相互作用诱导的CAT活性抑制无法捕获CAT的合成，因此导致活性增加和ROS水平升高。进一步的分子实验表明，SDS倾向于结合界面，而对CAT分子的活性位点和血红素基团的结构没有直接影响。当界面中的位点饱和时，SDS与VAL 73，HIS 74，ASN 147和PHE 152（酶活性的关键残基）相互作用，并导致CAT活性降低。 （C）2015 Elsevier B.V.保留所有权利。

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  • 来源
    《Journal of Hazardous Materials》 |2016年第15期|173-183|共11页
  • 作者

    Wang Jing; Wang Jiaxi; Xu Chi; Liu Rutao; Chen Yadong;

  • 作者单位

    Shandong Univ, Sch Environm Sci & Engn, China Amer CRC Environm & Hlth, 27 Shanda South Rd, Jinan 250100, Shandong, Peoples R China;

    Shandong Univ, Sch Environm Sci & Engn, China Amer CRC Environm & Hlth, 27 Shanda South Rd, Jinan 250100, Shandong, Peoples R China;

    Shandong Univ, Sch Environm Sci & Engn, China Amer CRC Environm & Hlth, 27 Shanda South Rd, Jinan 250100, Shandong, Peoples R China;

    Shandong Univ, Sch Environm Sci & Engn, China Amer CRC Environm & Hlth, 27 Shanda South Rd, Jinan 250100, Shandong, Peoples R China;

    China Pharmaceut Univ, Sch Basic Sci, Lab Mol Design & Drug Discovery, 24 Tongjiaxiang, Nanjing 210009, Jiangsu, Peoples R China;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Sodium dodecyl sulfate; Oxidative stress; Catalase; Isothermal titration calorimetry; Multi-spectroscopic studies;

    机译：十二烷基硫酸钠;氧化应激;过氧化氢酶;等温滴定量热法;多光谱研究;

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