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Evaluation of collagen/heparin coated TCP/HA granules for long-term delivery of BMP-2

机译：评估胶原蛋白/肝素包被的TCP / HA颗粒对BMP-2的长期递送

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Bone morphogenetic proteins (BMPs) are the most potent osteoinductive growth factors. However, a delivery system is essential to take advantage of the osteoinductive effect of BMPs. The purpose of this study was to develop a sustained delivery system for recombi-nant human bone morphogenetic protein-2 (BMP-2). We covalently attached heparin to a cross-linked collagen type I coated tricalciumphosphate/hydroxyapatite (TCP/HA) bone substitute and subsequently loaded it with BMP-2. To systematically evaluate the contribution of each component with respect to the binding and release of BMP-2, six constructs were prepared and characterized: TCP/HA, TCP/HA with collagen (TCP/HACol), and TCP/HA with collagen and heparin (TCP/HAColHep) with and without BMP-2 (B). More BMP-2 bound to the TCP/HAColHep + B (92.9 ± 4.8 ng BMP-2/mg granule) granules as compared to the TCP/HACol + B (69.0 ± 9.6 ng BMP-2/mg granule) and TCP/HA + B granules (62.9 ± 5.4 ng BMP-2/mg granule). No difference in release pattern was found between the TCP/HA + B and TCP/HACol + B granules. Up to day 14, BMP-2 was still bound to the TCP/ HAColHep + B granules, whereas most BMP had been released from TCP/HACol + B and TCP/HA + B granules at that time. After 21 days most BMP-2 also had been released from the TCP/HAColHep + B granules. The local and sustained delivery system for BMP-2 developed in this study may be useful as a carrier for BMP-2 and could possibly enhance bone regeneration efficacy for the treatment of large bone defects.
机译：骨形态发生蛋白（BMP）是最有效的骨诱导生长因子。但是，递送系统对于利用BMP的骨诱导作用至关重要。这项研究的目的是开发一种重组人骨形态发生蛋白2（BMP-2）的持续递送系统。我们将肝素共价连接到交联的I型胶原涂层的磷酸三钙/羟基磷灰石（TCP / HA）骨替代物上，然后将其装载BMP-2。为了系统地评估每种成分对BMP-2结合和释放的贡献，制备了六个构建体并进行了表征：TCP / HA，带有胶原蛋白的TCP / HA（TCP / HACol）和带有胶原蛋白和肝素的TCP / HA （TCP / HAColHep）使用和不使用BMP-2（B）。与TCP / HACol + B（69.0±9.6 ng BMP-2 / mg颗粒）和TCP / HA相比，更多的BMP-2与TCP / HAColHep + B（92.9±4.8 ng BMP-2 / mg颗粒）结合+ B颗粒（62.9±5.4 ng BMP-2 / mg颗粒）。在TCP / HA + B和TCP / HACol + B颗粒之间没有发现释放模式的差异。直到第14天，BMP-2仍与TCP / HAColHep + B颗粒结合，而那时大多数BMP已从TCP / HACol + B和TCP / HA + B颗粒中释放出来。 21天后，大多数BMP-2也已从TCP / HAColHep + B颗粒中释放出来。在这项研究中开发的BMP-2的局部和持续递送系统可能可用作BMP-2的载体，并可能增强骨骼再生功效，以治疗大的骨缺损。

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  • 来源
    《Journal of materials science》 |2013年第2期|325-332|共8页
  • 作者

    Gerjon Hannink; Paul J. Geutjes; Willeke F. Daamen; Pieter Buma;

  • 作者单位

    Orthopedic Research Laboratory, Department of Orthopedics, Radboud University Nijmegen Medical Centre, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands,Department of Operating Rooms, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands;

    Department of Urology, Radboud University Nijmegen Medical Centre, Nijmegen Centre for Molecular Life Sciences, Nijmegen, The Netherlands,Department of Biochemistry, Radboud University Nijmegen Medical Centre, Nijmegen Centre for Molecular Life Sciences, Nijmegen, The Netherlands;

    Department of Biochemistry, Radboud University Nijmegen Medical Centre, Nijmegen Centre for Molecular Life Sciences, Nijmegen, The Netherlands;

    Orthopedic Research Laboratory, Department of Orthopedics, Radboud University Nijmegen Medical Centre, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands;

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