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首页> 外文期刊>Journal of materials science >Peripheral mineralization of a 3D biodegradable tubular construct as a way to enhance guidance stabilization in spinal cord injury regeneration
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Peripheral mineralization of a 3D biodegradable tubular construct as a way to enhance guidance stabilization in spinal cord injury regeneration

机译：3D可生物降解的管状构造的周边矿化，以增强脊髓损伤再生中的引导稳定性

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摘要

Spinal cord injuries (SCI) present a major challenge to therapeutic development due to its complexity. Combinatorial approaches using biodegradable polymers that can simultaneously provide a tissue scaffold, a cell vehicle, and a reservoir for sustained drug delivery have shown very promising results. In our previous studies we have developed a novel hybrid system consisting of starch/poly-e-caprolactone (SPCL) semi-rigid tubular porous structure, based on a rapid prototyping technology, filled by a gellan gum hydrogel concentric core for the regeneration within spinal-cord injury sites. In the present work we intend to promote enhanced osteointegration on these systems by pre-mineralizing specifically the external surfaces of the SPCL tubular structures, though a biomi-metic strategy, using a sodium silicate gel as nucleating agent. The idea is to create two different cell environments to promote axonal regeneration in the interior of the constructs while inducing osteogenic activity on its external surface. By using a Teflon cylinder to isolate the interior of the scaffold, it was possible to observe the formation of a bone-like poorly crystalline carbonated apatite layer continuously formed only in the external side of the tubular structure. This biomimetic layer was able to support the adhesion of Bone Marrow Mesenchymal Stem Cells, which have gone under cytoskeleton reorganization in the first hours of culture when compared to cells cultured on uncoated scaffolds. This strategy can be a useful route for locally stimulate bone tissue regeneration and facilitating early bone ingrowth.
机译：脊髓损伤（SCI）由于其复杂性而对治疗发展提出了重大挑战。使用可同时提供组织支架，细胞载体和用于持续药物递送的储库的可生物降解聚合物的组合方法显示了非常有希望的结果。在我们以前的研究中，我们基于快速成型技术，开发了一种由淀粉/聚己内酯（SPCL）半刚性管状多孔结构组成的新型混合系统，并由结冷胶水凝胶同心芯填充以在脊髓内再生绳损伤部位。在本工作中，我们打算通过仿生策略，使用硅酸钠凝胶作为成核剂，通过专门矿化SPCL管状结构的外表面来促进这些系统上的骨整合。这个想法是创造两个不同的细胞环境，以促进构建体内部的轴突再生，同时在其外表面诱导成骨活性。通过使用特氟隆圆柱体来隔离支架的内部，可以观察到仅在管状结构的外侧连续形成的骨状的结晶性差的碳酸磷灰石层的形成。该仿生层能够支持骨髓间充质干细胞的粘附，与在未涂覆的支架上培养的细胞相比，该细胞在培养的最初几个小时就受到了细胞骨架的重组。该策略可能是局部刺激骨组织再生并促进早期骨长入的有用途径。

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  • 来源
    《Journal of materials science》 |2012年第11期|2821-2830|共10页
  • 作者

    A. L. Oliveira; E. C. Sousa; N. A. Silva; N. Sousa; A. J. Salgado; R. L. Reis;

  • 作者单位

    3B's Research Group-Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4806-909 Taipas, Guimaraes, Portugal,ICVS/3B's-PT Government Associate Laboratory, Braga/Guimaraes, Portugal,Department of Health Sciences, Portuguese Catholic University, Viseu, Portugal;

    3B's Research Group-Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4806-909 Taipas, Guimaraes, Portugal,ICVS/3B's-PT Government Associate Laboratory, Braga/Guimaraes, Portugal;

    3B's Research Group-Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4806-909 Taipas, Guimaraes, Portugal,ICVS/3B's-PT Government Associate Laboratory, Braga/Guimaraes, Portugal,Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Braga, Portugal;

    ICVS/3B's-PT Government Associate Laboratory, Braga/Guimaraes, Portugal,Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Braga, Portugal;

    ICVS/3B's-PT Government Associate Laboratory, Braga/Guimaraes, Portugal,Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Braga, Portugal;

    3B's Research Group-Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4806-909 Taipas, Guimaraes, Portugal;

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