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首页> 外文期刊>The Journal of Membrane Biology >Leucine Transport Is Affected by Bacillus thuringiensis Cry1 Toxins in Brush Border Membrane Vesicles from Ostrinia nubilalis Hb (Lepidoptera: Pyralidae) and Sesamia nonagrioides Lefebvre (Lepidoptera: Noctuidae) Midgut
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Leucine Transport Is Affected by Bacillus thuringiensis Cry1 Toxins in Brush Border Membrane Vesicles from Ostrinia nubilalis Hb (Lepidoptera: Pyralidae) and Sesamia nonagrioides Lefebvre (Lepidoptera: Noctuidae) Midgut

机译：苏云金芽孢杆菌Cry1毒素影响了白蛋白的运输，这些蛋白来自于Nostrinia nubilalis Hb（鳞翅目：Pyralidae）和Sesamia nonagrioides Lefebvre（鳞翅目：Noctuidae）中肠的刷状缘膜囊泡。

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摘要

The pore-forming activity of Cry1Ab, Cry1Fa and Cry1Ca toxins and their interaction with leucine transport mediated by the K+/leucine cotransporter were studied in brush border membrane vesicles (BBMVs) isolated from the midgut of Ostrinia nubilalis and Sesamia nonagrioides. In both species, as in other Lepidoptera, leucine uptake by BBMVs can take place in the absence of cations, but it can also be driven by a K+ gradient. Experiments with the voltage-sensitive fluorescent dye 3,3′-diethylthiacarbocyanine iodide proved that Cry1Ab, a Bacillus thuringiensis toxin active in vivo, enhanced the membrane permeability to potassium in O. nubilalis BBMVs. This result is in agreement with similar effects observed in S. nonagrioides BBMV incubated with various Cry1 toxins active in vivo. The effect of the above toxins was tested on the initial rate of 0.1 mM leucine influx. Instead of an increase in leucine influx, a reduction was observed with the Cry1 toxins active in vivo. Cry1Ab and Cry1Fa, but not the inactive toxin Cry1Da, inhibited in a dose-dependent manner leucine uptake both in the absence and in the presence of a K+ gradient, a clear indication that their effect is independent of the channel formed by the toxins and that this effect is exerted directly on the amino acid transport system.
机译：研究了从猪骨灰质中肠和半生芝麻中肠分离的刷状缘膜囊泡（BBMVs）中研究了Cry1Ab，Cry1Fa和Cry1Ca毒素的成孔活性及其与K + /亮氨酸共转运蛋白介导的亮氨酸转运的相互作用。在这两个物种中，如同在其他鳞翅目中一样，BBMVs可以在没有阳离子的情况下摄取亮氨酸，但也可以由K + 梯度驱动。用电压敏感型荧光染料3,3'-二乙基硫代卡巴菁碘化碘进行的实验证明，苏云金芽孢杆菌毒素在体内具有活性，Cry1Ab增强了O. nubilalis BBMVs对钾的膜通透性。该结果与在用多种在体内具有活性的Cry1毒素孵育的农杆菌链球菌BBMV中观察到的类似效果一致。测试了上述毒素对0.1 mM亮氨酸流入的初始速率的影响。观察到在体内有活性的Cry1毒素减少，而不是亮氨酸流入增加。 Cry1Ab和Cry1Fa，但无活性的毒素Cry1Da，在不存在和存在K +梯度的情况下均以剂量依赖的方式抑制亮氨酸的摄取，这清楚地表明它们的作用独立于由Cry1Da形成的通道。毒素，并且这种作用直接作用于氨基酸转运系统。

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