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首页> 外文期刊>Journal of Parasitic Diseases >Multiplex PCR assay using SCAR primers to detect Eimeria spp. in chicken
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Multiplex PCR assay using SCAR primers to detect Eimeria spp. in chicken

机译：使用SCAR引物的多重PCR检测法检测艾美尔球菌。在鸡里

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摘要

About 11 faecal samples from various regions of Tamil Nadu and Andhra Pradesh containing mixed spectrum of Eimeria species detected by morphometry viz. 15.4 × 11.2, 28.5 × 20.3, 31.1 × 18.5, 13.2 × 12.4, 20.8 × 17.5 and 22 × 18 μm for E. acervulina, E. brunetti, E. maxima, E. mitis, E. necatrix, E. praecox and E. tenella respectively were taken for the study. The oocysts were concentrated and purified using faecal harvest protocol. The genomic DNA is extracted as per kit protocol. The amplicons of sizes 539 bp (E. tenella) and 460 bp (E. mitis) obtained could be visualised in a single lane for the multiplex PCR assay using sequence characterized amplified region primers.
机译：来自泰米尔纳德邦和安得拉邦各个地区的大约11个粪便样品中含有形态测定法检测到的艾美球虫属混合光谱。对于小肠埃希菌，大肠埃希菌，大肠埃希菌，大肠埃希菌，大肠埃希菌，大肠埃希氏杆菌，大肠埃希菌和大肠埃希菌，15.4×11.2、28.5×20.3、31.1×18.5、13.2×12.4、20.8×17.5和22×18μm。 Tenella分别用于研究。使用粪便收集方案浓缩和纯化卵囊。按照试剂盒方案提取基因组DNA。获得的大小为539 bp（ten。E. tenella）和460 bp（E. mitis）的扩增子可以在单泳道中显示，以便使用序列特征性扩增区引物进行多重PCR分析。

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  • 来源
    《Journal of Parasitic Diseases》 |2013年第1期|110-113|共4页
  • 作者

    P. Venkateswara Rao; M. Raman; G. DhinakarRaj; S. Abdul Basith; S. Gomathinayagam;

  • 作者单位

    Department of Veterinary Parasitology Madras Veterinary College Tamil Nadu Veterinary Animal Sciences University">(1);

    Department of Veterinary Parasitology College of Veterinary Science Sri Venkateswara Veterinary University">(3);

    Department of Veterinary Parasitology Madras Veterinary College Tamil Nadu Veterinary Animal Sciences University">(1);

    Department of Animal Biotechnology Madras Veterinary College Tamil Nadu Veterinary Animal Sciences University">(2);

    Department of Veterinary Parasitology Madras Veterinary College Tamil Nadu Veterinary Animal Sciences University">(1);

    Department of Veterinary Parasitology Madras Veterinary College Tamil Nadu Veterinary Animal Sciences University">(1);

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    Mixed spectrum; Eimeria; Morphometry; Faecal harvest; Multiplex PCR; SCAR;

    机译：混合频谱;艾美球虫;形态计量学粪便收获;多重PCR;瘢痕;

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