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首页> 外文期刊>Journal of Parasitology >COMPARISON OF GENE REPRESENTATION BETWEEN DIPLOID AND TRIPLOID PARAGONIMUS WESTERMANI BY EXPRESSED SEQUENCE TAG ANALYSES
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COMPARISON OF GENE REPRESENTATION BETWEEN DIPLOID AND TRIPLOID PARAGONIMUS WESTERMANI BY EXPRESSED SEQUENCE TAG ANALYSES

机译：表达序列标签分析比较西双版氏和双下杆状对虾的基因表达

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摘要

Expressed sequence tag (EST) analysis of the diploid and triploid Paragonimus westermani genes was done to have a rapid and informative outlook of the gene-expression profiles of the parasites. Totals of 506 and 505 ESTs were generated from the diploid and triploid P. westermani cDNA libraries. Based on the BLASTx search results of the diploid P. westermani ESTs, 308 (60.9%) matched significantly with formerly identified genes and 198 (39.1%) showed no significant homology in the GenBank database. A similar homology pattern was shown from the triploid EST BLASTx search results with 346 (68.5%) sharing homology with previously identified genes and 159 (31.5%) showing no significant homology. The EST data from both libraries were analyzed and grouped into 9 categories. Comparison of the 2 EST pools revealed high similarities among the categories of the significantly matched genes. Single genes matched repeatedly were also observed in the 2 EST data. Some genes were found that are not yet characterized in P. westermani; these genes were matched by both the diploid and triploid ESTs. Further study of these genes may provide us with more understanding on the parasite's biology and their specific functions in the 2 strains.
机译：进行了对二倍体和三倍体百日咳对虾基因的表达序列标签（EST）分析，以快速，丰富地了解该寄生虫的基因表达谱。从二倍体和三倍体P. westermani cDNA文库中总共产生了506和505个EST。根据二倍体中国小麦EST的BLASTx搜索结果，有308（60.9％）个与以前鉴定的基因显着匹配，而198个（39.1％）在GenBank数据库中显示无显着同源性。从三倍体EST BLASTx搜索结果中显示了相似的同源性模式，其中346（68.5％）与先前鉴定的基因共有同源性，而159（31.5％）没有显着同源性。分析两个库中的EST数据并将其分为9类。 2个EST池的比较显示出显着匹配的基因类别之间的高度相似性。在2个EST数据中也观察到了重复匹配的单个基因。发现了一些尚未在韦氏疟原虫中表征的基因；这些基因与二倍体和三倍体EST都匹配。这些基因的进一步研究可能使我们对2种菌株中的寄生虫生物学及其特定功能有更多的了解。

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