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首页> 外文期刊>Journal of Parasitology >DETECTION OF BLASTOCYSTIS HOMINIS IN UNPRESERVED STOOL SPECIMENS BY USING POLYMERASE CHAIN REACTION
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DETECTION OF BLASTOCYSTIS HOMINIS IN UNPRESERVED STOOL SPECIMENS BY USING POLYMERASE CHAIN REACTION

机译：聚合酶链反应法检测未保留样品中的猪瘟菌

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摘要

Blastocystis hominis is a common enteric parasite of worldwide distribution. Its pathogenetic potential has not yet been established, although numerous case reports suggest that B. hominis may cause the development of various gastrointestinal symptoms and disorders. The detection of the parasite in stool specimens is conventionally done by microscopy of direct smears, fecal concentrates, or permanently stained smears; however, morphology-based diagnosis is problematic. The aim of this study was to develop and evaluate a polymerase chain reaction (PCR) technique for the direct detection of B. hominis in human stool samples. Primers were based on small subunit ribosomal DNA and able to detect 32 parasites/200 mg stool artificially spiked with cultured B. hominis. In the evaluation of 43 clinical specimens, the PCR was tested against the formol ethyl acetate concentration technique (FECT) and a culture technique, proving 100% test specificity and a significantly higher sensitivity than the FECT. The PCR method is recommended for screening clinical specimens for B. hominis infection and for use in prevalence studies.
机译：人型芽孢杆菌是全世界分布的常见肠寄生虫。尽管许多病例报告表明人型芽孢杆菌可能引起各种胃肠道症状和疾病，但其致病潜力尚未确定。粪便标本中的寄生虫的检测通常通过直接涂片，粪便浓缩物或永久性涂片的显微镜检查来完成。然而，基于形态学的诊断是有问题的。这项研究的目的是开发和评估可直接检测人粪便样本中人双歧杆菌的聚合酶链反应（PCR）技术。引物基于小的亚基核糖体DNA，能够检测到人工培养的人双歧杆菌的32种寄生虫/ 200 mg粪便。在对43个临床标本的评估中，针对甲酚乙酸乙酯浓缩技术（FECT）和培养技术对PCR进行了测试，证明了100％的测试特异性和明显高于FECT的灵敏度。建议使用PCR方法筛查人源双歧杆菌感染的临床标本并用于患病率研究。

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