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首页> 外文期刊>Journal of Parasitology >MOLECULAR CHARACTERIZATION OF THE 18S rDNA GENE OF AN AVIAN HEPATOZOON REVEALS THAT IT IS CLOSELY RELATED TO LANKESTERELLA
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MOLECULAR CHARACTERIZATION OF THE 18S rDNA GENE OF AN AVIAN HEPATOZOON REVEALS THAT IT IS CLOSELY RELATED TO LANKESTERELLA

机译：与兰科氏菌密切相关的禽类肝祖虫揭示物的18S rDNA基因的分子表征

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摘要

As a part of intensive study of blood parasite infections in a population of the passerine bird blue tit (Cyanistes caeruleus, Paridae), we detected a parasite species that, based on its morphological similarity, was tentatively identified as Hepatozoon parus, the only species of this parasite genus described from birds of this family. However, morphological measurements show that H. parus is slightly larger than the parasite detected in our population. A molecular characterization of the parasite species was conducted by amplification of the 18S rDNA gene, using primers that were reported previously to amplify in Hepatozoon sp. of water pythons. Additional primers were developed based on the new sequence obtained. The 1,484-bp fragment amplified reveals that the parasite from our bird population is more closely related to Lankesterella minima than to Hepatozoon species from other vertebrates according to analysis using the BLAST comparison method (93% identity). In addition, phylogenetic analyses using parsimony and Kimura procedures unequivocally related the parasite species detected by PCR with L. minima. The bootstrap values obtained were 97% and 100%, respectively. These results imply that this parasite is a species of a lankesterellid instead of Hepatozoon.
机译：作为对雀形目鸟蓝雀（Cyanistes caeruleus，Paridae）种群中血液寄生虫感染进行深入研究的一部分，我们检测到了一种寄生虫物种，根据其形态相似性，暂时将其鉴定为Hepatozoon parus，是该物种的唯一物种。从该家族的鸟类中描述的这种寄生虫属。但是，形态学测量表明，H。parus稍大于我们种群中检测到的寄生虫。使用以前报道的在Hepatozoon sp。中扩增的引物，通过18S rDNA基因的扩增来进行寄生虫物种的分子表征。水蟒。根据获得的新序列开发其他引物。根据使用BLAST比较方法进行的分析（93％一致性），扩增的1,484 bp片段揭示出，与其他脊椎动物的Hepatozoon物种相比，来自我们鸟类的寄生虫与最小化的Lankesterella物种更紧密相关。另外，使用简约和木村方法进行的系统发育分析明确地将PCR检测到的寄生虫与最小乳杆菌相关联。获得的自举值分别为97％和100％。这些结果暗示该寄生虫是兰科氏菌而不是肝菌的种类。

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