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首页> 外文期刊>Journal of Parasitology >ECTO- AND EXO-PROTEIN KINASES IN SCHISTOSOMA MANSONI: REGULATION OF SURFACE PHOSPHORYLATION BY ACETYLCHOLINE AND IDENTIFICATION OF THE ALPHA SUBUNIT OF CKII AS A MAJOR SECRETED PROTEIN KINASE
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ECTO- AND EXO-PROTEIN KINASES IN SCHISTOSOMA MANSONI: REGULATION OF SURFACE PHOSPHORYLATION BY ACETYLCHOLINE AND IDENTIFICATION OF THE ALPHA SUBUNIT OF CKII AS A MAJOR SECRETED PROTEIN KINASE

机译：曼氏血吸虫中的ecto和exo蛋白激酶：乙酰胆碱对表面磷酸化的调节和作为主要分泌蛋白激酶的CKIIα亚基的鉴定

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The Schistosoma mansoni parasite life cycle involves complex developmental processes that enable it to cause severe hepatic damage. Protein phosphorylation has previously been implicated in the transformation of cercariae to schistosomula of S. mansoni. Here, we studied the possible involvement of surface (ecto) and shed (exo) protein kinases (PKs) in this developmental process. We found that ecto-PKs are indeed located on the surface of the schistosomula and can phosphorylate up to 5 distinct proteins at this location. Surface phosphorylation was sensitive to acetylcholine, which increased phosphorylation of 3 proteins and reduced phosphorylation of the other 2. The ecto-PKs can be shed from the surface into the incubation medium during parasite differentiation. The main exo-PK is CKII, as concluded from the substrate specificity of the PK, its inhibition by heparin, activation by spermin, and recognition by antibody directed to the anti–α-subunit of CKII in the incubation medium of the schistosomula. In spite of its similarity to the ecto-PKs, the activity of the exo-PK is not affected by addition of acetylcholine. These results indicate that ecto- and exo-PKs could be involved in the parasite's development or host–parasite interactions.
机译：曼氏血吸虫寄生虫的生命周期涉及复杂的发育过程，使其能够引起严重的肝损伤。先前已经将蛋白质磷酸化与尾c向曼氏链球菌的血吸虫的转化有关。在这里，我们研究了表面（外在）和脱落（外在）蛋白激酶（PKs）在此发育过程中的可能参与。我们发现ecto-PKs确实位于血吸虫的表面，并且可以在此位置磷酸化多达5种不同的蛋白质。表面磷酸化对乙酰胆碱敏感，乙酰胆碱增加了3种蛋白质的磷酸化而降低了其他2种蛋白质的磷酸化。在寄生虫分化过程中，胞外PKs可能从表面脱落到培养液中。主要的exo-PK是CKII，由PK的底物特异性，其对肝素的抑制作用，对精子的激活以及在血吸虫培养液中针对CKII的抗α亚基的抗体的识别得出。尽管其与胞外PKs相似，但加入乙酰胆碱不会影响exo-PK的活性。这些结果表明，外PK和外PK可能与寄生虫的发育或宿主-寄生虫的相互作用有关。

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    《Journal of Parasitology》 |2005年第4期|p.00000756-00000763|共8页
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    Rebeca Tarrab-Hazdai* Tamar Hanoch† Sung Ho Jeon Henya Pauli‡ Daniel Gold‡ Ruth Arnon and Rony Seger†§;

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    Department of Immunology, The Weizmann Institute of Science 76100 Rehovot, Israel. rony.seger@weizmann.ac.il*This article is dedicated to the memory of Dr. Rebeca Tarrab-Hazdai, deceased, October 2001†Department of Biological Regulation, The Weizman Institute of Science, 761090 Rehovot, Israel‡The Department of Human Microbiology, Faculty of Medicine, Tel Aviv University, Tel Aviv 69978, Israel§To whom correspondence should be addressed;

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