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首页> 外文期刊>Journal of Parasitology >A POLARIZED TH1 TYPE IMMUNE RESPONSE TO COWDRIA RUMINANTIUM INFECTION IS DETECTED IN IMMUNE DBA/2 MICE
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A POLARIZED TH1 TYPE IMMUNE RESPONSE TO COWDRIA RUMINANTIUM INFECTION IS DETECTED IN IMMUNE DBA/2 MICE

机译：在免疫DBA / 2小鼠中检测到了对CowDRIA RuNantium感染的极化TH1型免疫反应。

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Immune responses to Cowdria ruminantium, an intracellular organism that causes heartwater in domestic ruminants, were characterized in a DBA/2 mouse model. Immunity induced by infection and treatment was adoptively transferable by splenocytes and could be abrogated by in vivo depletion of T cells but not by inhibition of nitric oxide synthase using NG-monomethyl-l-arginine. IgG2a and IgG2b C. ruminantium-specific responses were detected in immune mice. Culture supernatants of splenocytes from immune DBA/2 mice, which were stimulated with crude C. ruminantium antigens or recombinant major antigenic proteins 1 or 2, contained significant levels of interferon (IFN)-γ and interleukin (IL)-6, but insignificant levels of IL-1α, IL-2, IL-4, IL-5, IL-10, IL-12, tumor necrosis factor-α (TNF), and nitric oxide. A similar response was detected during primary infection, although IFN-γ levels decreased significantly during clinical illness and then increased following natural or antibiotic-aided recovery. These data support the conclusion that protective immunity to C. ruminantium in DBA/2 mice is mediated by T cells and is associated with a polarized T helper 1 type of immune response. This murine model could be utilized to screen for protective C. ruminantium antigens that provoke Th1 type immune responses and for evaluation of these antigens in recombinant vaccines against heartwater.
机译：在DBA / 2小鼠模型中表征了对反刍动物反刍动物的免疫反应，反刍动物是一种会引起反刍动物心水的细胞内生物。感染和治疗引起的免疫力可以通过脾细胞过继转移，并且可以通过体内T细胞的消耗而被废除，但不能通过使用NG-单甲基-1-精氨酸抑制一氧化氮合酶而被废除。在免疫小鼠中检测到了IgG2a和IgG2b反刍类反刍动物特异性反应。来自免疫DBA / 2小鼠的脾细胞的培养上清液受粗制的反刍动物抗原或重组主要抗原蛋白1或2刺激，含有大量干扰素（IFN）-γ和白介素（IL）-6，但水平不高IL-1α，IL-2，IL-4，IL-5，IL-10，IL-12，肿瘤坏死因子-α（TNF）和一氧化氮的含量。尽管在临床疾病期间IFN-γ水平显着降低，然后在自然或抗生素辅助恢复后升高，但在初次感染期间也检测到类似的反应。这些数据支持这样的结论，即DBA / 2小鼠对反刍动物的保护性免疫是由T细胞介导的，并且与极化的T辅助1型免疫反应有关。该鼠模型可用于筛选引发Th1型免疫反应的保护性反刍类反刍动物抗原，并在针对心水的重组疫苗中评估这些抗原。

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