• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Polymers and the Environment >A Novel Bacterial Polymeric Silk-Like Protein from a Petroleum Origin Bacillus sp. strain NE: Isolation and Characterization
【24h】

A Novel Bacterial Polymeric Silk-Like Protein from a Petroleum Origin Bacillus sp. strain NE: Isolation and Characterization

机译：一种来自石油来源的新型细菌聚合物丝状蛋白质芽孢杆菌。菌株NE：隔离和表征

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

This paper explores a novel silk-like protein (named Bacillus NE silk (BNES)) isolated from a petroleum origin bacterial strain belongs to the genus Bacillus. The successful characterizations for its chemical structure determination and protein composition have been performed. The results demonstrate that BNES protein is a new type of silk-like protein and has a prospective for applying as a new bacterial silk-like biomaterial. The amino acids constituents of silk-like protein showed that (Ala-Pro-Gly) rich-block protein, are the main major amino acids, while the protein M-wt., was recorded aroundsimilar to 400kDa, which excelled those similar of natural silk. The degummed or purified silk-like protein was successfully electro-spinned for wet-electrospun silk-like nanofibers using electrospinning technique. Silk-like nanofibers gave an adequate and uniform ribbon-shape structure with average diameter of nanofiber around 110nm. Meanwhile, the block silk-like protein exhibited high thermal degradation monitored at 140-373 degrees C, which close those of natural silkworm silk. The purified silk-like protein showed no toxic effect against Vero kidney normal cell-line even after 48h of incubation, using MTT-assay test. The obtained results demonstrate that, silk-like protein from wild type bacterial origin has potential for use as novel fibrous biomaterials for biomedical and environmental applications.
机译：本文探讨了从石油原产地的细菌菌株中分离的新型丝绸样蛋白（名为Bacillus Ne Silk（Bnes））属于芽孢杆菌属。已经进行了其化学结构测定和蛋白质组合物的成功表征。结果表明，BNE蛋白是一种新型的丝绸样蛋白，具有施用作为新的细菌丝状生物材料的前瞻性。丝状蛋白的氨基酸成分显示（Ala-pro-gly）富含嵌段蛋白，是主要的主要氨基酸，而蛋白质m-wt，则被记录在>类似于400kda，这使得类似于400kda天然丝绸。使用静电纺丝技术成功地将脱胶或纯化的丝绸状蛋白成功地电动 - 湿纺丝纳​​米纤维。丝状纳米纤维具有足够且均匀的带状结构，具有大约110nm的纳米纤维的平均直径。同时，块状丝状蛋白在140-373摄氏度监测的高热降解，靠自然蚕丝。纯化的丝绸样蛋白在使用MTT-Assay试验后，甚至在孵育48小时后，均对Vero肾正常细胞系没有毒性效果。所得结果表明，来自野生型细菌来源的丝状蛋白质具有用作生物医学和环境应用的新型纤维生物材料。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号