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首页> 外文期刊>Journal of Polymers and the Environment >A Novel Bacterial Polymeric Silk-Like Protein from a Petroleum Origin Bacillus sp. strain NE: Isolation and Characterization
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A Novel Bacterial Polymeric Silk-Like Protein from a Petroleum Origin Bacillus sp. strain NE: Isolation and Characterization

机译：从石油起源的芽孢杆菌属的一种新型细菌聚合丝样蛋白。 NE菌株：分离和鉴定

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摘要

This paper explores a novel silk-like protein (named Bacillus NE silk (BNES)) isolated from a petroleum origin bacterial strain belongs to the genus Bacillus. The successful characterizations for its chemical structure determination and protein composition have been performed. The results demonstrate that BNES protein is a new type of silk-like protein and has a prospective for applying as a new bacterial silk-like biomaterial. The amino acids constituents of silk-like protein showed that (Ala-Pro-Gly) rich-block protein, are the main major amino acids, while the protein M-wt., was recorded aroundsimilar to 400kDa, which excelled those similar of natural silk. The degummed or purified silk-like protein was successfully electro-spinned for wet-electrospun silk-like nanofibers using electrospinning technique. Silk-like nanofibers gave an adequate and uniform ribbon-shape structure with average diameter of nanofiber around 110nm. Meanwhile, the block silk-like protein exhibited high thermal degradation monitored at 140-373 degrees C, which close those of natural silkworm silk. The purified silk-like protein showed no toxic effect against Vero kidney normal cell-line even after 48h of incubation, using MTT-assay test. The obtained results demonstrate that, silk-like protein from wild type bacterial origin has potential for use as novel fibrous biomaterials for biomedical and environmental applications.
机译：本文探讨了一种新的丝状蛋白（称为芽孢杆菌NE丝（BNES）），该蛋白是从属于芽孢杆菌属的石油来源细菌菌株中分离出来的。已对其化学结构确定和蛋白质组成进行了成功的表征。结果表明，BNES蛋白是一种新型的丝状蛋白，具有作为新型细菌丝状生物材料的应用前景。丝状蛋白的氨基酸组成表明，（Ala-Pro-Gly）丰富的嵌段蛋白是主要的主要氨基酸，而M-wt。的蛋白记录> 400kDa左右，优于那些相似的蛋白。天然丝绸。使用静电纺丝技术将脱胶或纯化的丝状蛋白成功地电纺成湿静电纺丝状纳米纤维。丝状纳米纤维给出了足够均匀的带状结构，纳米纤维的平均直径约为110nm。同时，块状丝状蛋白在140-373℃下显示出高的热降解，这接近天然蚕丝。使用MTT测定法，即使在孵育48小时后，纯化的丝状蛋白也没有显示出对Vero肾脏正常细胞系的毒性作用。获得的结果表明，来自野生型细菌来源的丝状蛋白具有用作生物医学和环境应用的新型纤维生物材料的潜力。

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