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首页> 外文期刊>Journal of the American Chemical Society >COOPERATIVE TRIPLE-HELIX FORMATION AT ADJACENT DNA SITES - SEQUENCE COMPOSITION DEPENDENCE AT THE JUNCTION
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COOPERATIVE TRIPLE-HELIX FORMATION AT ADJACENT DNA SITES - SEQUENCE COMPOSITION DEPENDENCE AT THE JUNCTION

机译：相邻DNA站点上的协作三重螺旋形成-交界处的序列组成依赖

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The energetics of cooperative binding by oligodeoxyribonucleotides to adjacent sites by triple helix formation have been determined as a function of sequence composition at the junction. The binding affinity of an 11-mer in the presence of a neighboring bound oligonucleotide is enhanced by factors of 12, 17, 61, and 127 when 5'-TT-3', 5'-(CC)-C-m-C-m-3', 5'-(TC)-C-m-3', and 5'-(CT)-C-m-3' stacks, respectively, are formed at the junction (10 mM Bis-Tris . HCl at pH 7.0, 10 mM NaCl, 250 mu M spermine, 24 degrees C). These binding enhancements correspond to interaction energies between the two oligonucleotides of 1.5, 1.7, 2.5, and 2.9 kcal . mol(-1), respectively. The energetic penalties for a single-base mismatch differ depending on sequence and the location of the mismatch with respect to the 5'- or 3'-side of the junction. In the case of a 5'-TT-3' stack, a T . GC mismatch on the 5'-side of the junction decreases the interaction energy from 1.5 to 0.6 kcal . mol(-1), whereas a T . GC mismatch on the 3'-side destroys cooperativity. For a 5'-(CT)-C-m-3' stack, a C-m . AT mismatch on the 5'-side of the junction decreases the cooperative interaction energy from 2.9 to 1.7 kcal . mol(-1), whereas a T . GC mismatch on the 3'-side of the junction destroys cooperativity. Two 11-mer oligonucleotides interacting through a 5'-TT-3' stack binding to adjacent sites on DNA are significantly more sensitive to single-base mismatches than the corresponding 22-mer binding to the same two abutting sites. [References: 35]
机译：已经确定寡聚脱氧核糖核苷酸通过三重螺旋形成与相邻位点的协同结合的能量是在连接处的序列组成的函数。当5'-TT-3'，5'-（CC）-CmCm-3'，12'，17、61和127因子增强11-mer在相邻结合寡核苷酸存在下的结合亲和力在连接处分别形成5'-（TC）-Cm-3'和5'-（CT）-Cm-3'堆叠（pH 7.0的10 mM Bis-Tris.HCl，10 mM NaCl，250 μM精胺，24摄氏度）。这些结合增强对应于1.5、1.7、2.5和2.9kcal的两个寡核苷酸之间的相互作用能。 mol（-1）。单碱基不匹配的能量损失取决于序列和相对于连接的5'或3'侧的不匹配位置。如果是5'-TT-3'堆栈，则为T。结5'端的GC不匹配将相互作用能从1.5 kcal降低到0.6 kcal。 mol（-1），而T。 3'端的GC不匹配破坏了合作性。对于5'-（CT）-C-m-3'堆栈，为C-m。在结的5'侧的AT失配将协同相互作用能从2.9kcal降低至1.7kcal。 mol（-1），而T。结3'侧的GC不匹配破坏了协同性。通过与DNA相邻位点结合的5'-TT-3'堆栈相互作用的两个11-mer寡核苷酸对单碱基错配的敏感性比与相同的两个邻接位点的22-mer结合更为敏感。 [参考：35]

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