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Preparation of aligned poly(glycerol sebacate) fibrous membranes for anisotropic tissue engineering

机译：各向异性组织工程用取向聚癸二酸甘油酯纤维膜的制备

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摘要

The use of fibrous scaffolds for tissue repair or regeneration is advantageous for its microstructure similar to that of the native ECM. Aligned fibrous scaffold, in particular, can be used to manipulate cell alignment and hence the microstructure of the resultant tissue. In our previous study, nanofibers consisting of solely poly(glycerol sebacate) (PGS) have been successfully fabricated using core-shell coaxial electrospinning followed by curing and subsequent shell removal. When we tried to fabricate aligned PGS fibrous membranes by collecting the electrospun fibers on a rapidly rotating drum, however, loss of fibrous structure was observed upon curing. This might be due to the broken fibers that were collected under tension; the core PGS prepolymer that melts at high temperature could leak from the broken ends during curing. In this study, attempts were made to reduce the possibility of the fiber breakage. At each stage of preparation, fiber morphology was examined by SEM and fiber compositions were verified by Fourier transform infrared spectroscopy and differential scanning calorimetry. Mechanical properties of the aligned PGS fibrous membrane were evaluated by uniaxial tensile testing both in parallel and perpendicular to the principal fiber direction. SEM images showed that fibrous morphology was better preserved upon the adjustment of the shell composition and the rotational speed of the collector drum. The final PGS fibers remained to be aligned although the alignment was less strong than that of as-spun coreshell fibers. The aligned PGS fibrous membrane exhibited anisotropic mechanical properties with Young's modulus in parallel and perpendicular to the principal fiber direction being 0.98 +/- 0.04 MPa and 0.52 +/- 0.02 MPa, respectively. The aligned PGS fibrous membrane was capable of guiding the orientation of cultured cells and therefore has the potential to be used to fabricate structurally anisotropic tissue-engineered constructs.
机译：使用纤维状支架进行组织修复或再生是有利的，因为其微观结构类似于天然ECM。对齐的纤维支架尤其可以用于操纵细胞的对齐，从而控制所得组织的微观结构。在我们先前的研究中，已经成功地使用核-壳同轴电纺丝并随后固化和随后去除壳的方法成功地制造了仅由聚癸二酸甘油酯（PGS）组成的纳米纤维。但是，当我们尝试通过在快速旋转的鼓上收集电纺纤维来制作取向的PGS纤维膜时，在固化时会观察到纤维结构的损失。这可能是由于在张力作用下收集到的断裂纤维所致。在固化过程中，高温熔融的PGS核心预聚物可能会从断裂端泄漏。在这项研究中，试图减少纤维断裂的可能性。在制备的每个阶段，通过SEM检查纤维形态，并通过傅立叶变换红外光谱和差示扫描量热法验证纤维成分。取向的PGS纤维膜的机械性能通过平行于和垂直于主要纤维方向的单轴拉伸试验进行评估。 SEM图像显示，通过调节壳组成和收集器鼓的旋转速度，可以更好地保留纤维形态。最终的PGS纤维保持对准，尽管该对准强度不如初纺的核壳纤维强。取向的PGS纤维膜表现出各向异性的机械性能，平行和垂直于主纤维方向的杨氏模量分别为0.98 +/- 0.04MPa和0.52 +/- 0.02MPa。对齐的PGS纤维膜能够引导培养细胞的方向，因此具有用于制造结构各向异性的组织工程化构建体的潜力。

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