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首页> 外文期刊>Materials science & engineering >Multi-walled carbon nanotube carpets as scaffolds for U87MG glioblastoma multiforme cell growth
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Multi-walled carbon nanotube carpets as scaffolds for U87MG glioblastoma multiforme cell growth

机译：多壁碳纳米管地毯作为U87MG胶质母细胞瘤多形细胞生长的支架

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Carbon Nanotubes (CNTs) are known for effective adhesion, growth, and differentiation of bone, muscle, and cardiac cells. CNTs can provide excellent mechanical and electrical properties for cell scaffolding; however, loose CNTs can cause in-vivo toxicity. To suppress this risk, our team has developed biomimetic scaffolds with multiscale hierarchy where carpet-like CNT arrays are covalently bonded to larger biocompatible substrates. In this study, we investigated the interaction between glioblastoma multiforme (GBM) cells (U87MG) and our unique hierarchical CNT-coated scaffolds upon brain tumor cell proliferation. U87MG cells grown on unmodified carbon scaffolds grew in a bi-phasic fashion. Initially, the scaffolds prevented GBM cell growth; however, prolonged growth on such scaffolds significantly increased GBM cell proliferation. We further defined the importance of the hydrophobicity/hydrophilicity of the CNT-coated scaffolds in this cellular response by utilizing sodium-hypochlorite based bleach treatment prior to cellular exposure. This surface modification increased the hydrophilicity of the CNT-coated scaffolds and ameliorated the biphasic response of U87MG cells allowing for a normal growth curve. Findings highlight the importance of surface modification and wettability of the CNT-coated scaffolds for cell growth applications. The focus for this study was to determine whether scaffold surface features could modulate tumor-scaffold interactions, and thus to improve our understanding of and optimize successful development of future scaffold-based chemotherapy applications. Overall, it appears that the wettability of carbon scaffolds coated with CNTs is an important regulator of U87MG cellular growth. These findings will be important to consider when developing a potential chemotherapy-attached implant to be used post-surgical resection for GBM patient treatment.
机译：碳纳米管（CNT）可以有效地粘附，生长和分化骨骼，肌肉和心肌细胞。 CNT可为细胞支架提供出色的机械和电气性能;但是，疏松的碳纳米管会引起体内毒性。为了抑制这种风险，我们的团队开发了具有多尺度层次的仿生支架，其中地毯状CNT阵列与较大的生物相容性基质共价键合。在这项研究中，我们调查了脑胶质母细胞瘤（GBM）细胞（U87MG）与我们独特的分层CNT涂层支架在脑肿瘤细胞增殖之间的相互作用。在未经修饰的碳支架上生长的U87MG细胞以双相方式生长。最初，支架阻止了GBM细胞的生长。然而，在此类支架上的长期生长显着增加了GBM细胞的增殖。我们通过在细胞暴露前利用基于次氯酸钠的漂白剂处理进一步定义了碳纳米管包覆的支架的疏水性/亲水性在这种细胞反应中的重要性。这种表面修饰增加了CNT涂层支架的亲水性，并改善了U87MG细胞的双相反应，从而允许正常的生长曲线。研究结果突显了碳纳米管涂覆的支架表面改性和润湿性对于细胞生长应用的重要性。这项研究的重点是确定支架表面特征是否可以调节肿瘤-支架相互作用，从而增进我们对未来基于支架的化学疗法应用的理解并优化其成功开发。总的来说，涂有碳纳米管的碳支架的润湿性似乎是U87MG细胞生长的重要调节剂。在开发可能用于化疗的GBM患者手术后切除的植入式化学植入物时，这些发现将是重要的考虑因素。

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    《Materials science & engineering》 |2020年第3期|110345.1-110345.10|共10页
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    Wright State Univ Dept Mech & Mat Engn Ctr Nanoscale Multifunct Mat 3640 Col Glen Hwy Dayton OH 45435 USA|Wright State Univ Translat Neurosci Inst Dept Neurosci Cell Biol & Physiol Boonshoft Sch Med Coll Sci & Math 3640 Col Glen Hwy Dayton OH 45435 USA;

    Wright State Univ Translat Neurosci Inst Dept Neurosci Cell Biol & Physiol Boonshoft Sch Med Coll Sci & Math 3640 Col Glen Hwy Dayton OH 45435 USA;

    Wright State Univ Dept Mech & Mat Engn Ctr Nanoscale Multifunct Mat 3640 Col Glen Hwy Dayton OH 45435 USA;

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