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首页> 外文期刊>Materials science & engineering >Understanding the influence of phosphorylation and polysialylation of gelatin on mineralization and osteogenic differentiation
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Understanding the influence of phosphorylation and polysialylation of gelatin on mineralization and osteogenic differentiation

机译：了解明胶的磷酸化和多唾液酸化对矿化和成骨分化的影响

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摘要

Post-translational modifications such as phosphorylation and sialylation impart crucial functions such as mineral deposition and osteogenic differentiation to non-collagenous bone matrix proteins. In this work, the influence of phosphorylation and polysialylation of gelatin on mineralization in simulated body fluid (SBF) and on osteogenic differentiation of mesenchymal stem cells (MSC) was studied. It was observed that increase in phosphorylation could be directly correlated with the mineralization ability of phosphorylated gelatin in SBF. The total calcium and phosphate deposited increased with increase in degree of phosphorylation and was ＞3 fold higher on the highest degree of phosphorylation. Whereas, polysialylation did not have any significant influence on mineral deposition in SBF. On the other hand, when MSCs were cultured on polysialylated surfaces they showed relatively higher cell elongation with 1.5 fold higher cell aspect ratio, higher alkaline phosphatase activity and 3 fold higher mineral deposition when compared to control and phosphorylated gelatin surfaces. In conclusion, phosphorylation and polysialylation of gelatin show a significant influence on mineralization and osteogenic differentiation respectively which can be advantageously used for bone tissue engineering.
机译：翻译后修饰（例如磷酸化和唾液酸化）赋予非胶原骨基质蛋白重要的功能，例如矿物质沉积和成骨分化。在这项工作中，研究了明胶的磷酸化和多唾液酸化对模拟体液（SBF）中矿化和对间充质干细胞（MSC）成骨分化的影响。观察到磷酸化的增加可能与SBF中磷酸化明胶的矿化能力直接相关。沉积的总钙和磷酸盐随着磷酸化程度的增加而增加，并且在最高的磷酸化程度下高＞ 3倍。而聚唾液酸化对SBF中的矿物质沉积没有显着影响。另一方面，当将MSCs培养在多唾液酸化的表面上时，与对照和磷酸化的明胶表面相比，它们显示出相对更高的细胞伸长率，具有1.5倍的细胞长宽比，更高的碱性磷酸酶活性和3倍的矿物质沉积。总之，明胶的磷酸化和聚唾液酸化分别显示出对矿化和成骨分化的显着影响，可有利地用于骨组织工程。

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