...
首页> 外文期刊>Materials science & engineering >Surface microcracks signal osteoblasts to regulate alignment and bone formation
【24h】

Surface microcracks signal osteoblasts to regulate alignment and bone formation

机译:表面微裂纹向成骨细胞发出信号以调节排列和骨形成

获取原文
获取原文并翻译 | 示例
           

摘要

Microcracks are present in bone and can result from fatigue damage due to repeated, cyclically applied stresses. From a mechanical point, microcracks can dissipate strain energy at the advancing tip of a crack to improve overall bone toughness. Physiologically, microcracks are thought to trigger bone remodeling. Here, we examine the effect of microcracks specifically on osteoblasts, which are bone-forming cells, by comparing cell responses on microcracked versus non-microcracked hydroxyapatite (HA) specimens. Osteoblast attachment was found to be greater on microcracked HA specimens (p < 0.05). More importantly, we identified the preferential alignment of osteoblasts in the direction of the microcracks on HA. Cells also displayed a preferential attachment that was 75 to 90 μm away from the microcrack indent After 21 days of culture, osteoblast maturation was notably enhanced on the HA with microcracks, as indicated by increased alkaline phosphatase activity and gene expression. Furthermore, examination of bone deposition by confocal laser scanning microscopy indicated preferential mineralization at microcrack indentation sites. Dissolution studies indicate that the microcracks increase calcium release, which could contribute to osteoblast responses. Our findings suggest that microcracks signal osteoblast attachment and bone formation/healing.
机译:微裂纹存在于骨骼中,可能是由于反复循环施加应力而造成的疲劳损坏。从机械角度来看,微裂纹可以消散裂纹前进尖端的应变能,从而改善整体骨骼韧性。在生理上,微裂纹被认为可以触发骨骼重塑。在这里,我们通过比较微裂纹和非微裂纹的羟基磷灰石(HA)标本上的细胞反应,来检查微裂纹对成骨细胞(成骨细胞)的影响。发现微裂纹HA标本中成骨细胞的附着力更大(p <0.05)。更重要的是,我们确定了成骨细胞在HA微裂纹方向上的优先排列。细胞还显示出与微裂纹凹痕相距75至90μm的优先附着。培养21天后,微裂纹使HA上的成骨细胞成熟显着增强,这表现为碱性磷酸酶活性和基因表达增加。此外,通过共聚焦激光扫描显微镜检查的骨沉积表明在微裂纹压痕部位优先矿化。溶出度研究表明,微裂纹会增加钙的释放,这可能有助于成骨细胞的反应。我们的发现表明,微裂纹表明成骨细胞附着和骨形成/愈合。

著录项

  • 来源
    《Materials science & engineering》 |2014年第11期|191-200|共10页
  • 作者单位

    Chemical Engineering and Materials Science Department, Michigan State University, United States,The College of Materials Science and Engineering, Beijing University of Technology, Beijing 100124, People's Republic of China;

    Chemical Engineering and Materials Science Department, Michigan State University, United States,Department of Mechanical Engineering, Auburn University, Auburn, AL 36849, United States;

    Chemical Engineering and Materials Science Department, Michigan State University, United States;

    Department of Physiology, Michigan State University, East Lansing, MI 48824, United States,Department of Radiology, Michigan State University, East Lansing, MI 48824, United States;

    Chemical Engineering and Materials Science Department, Michigan State University, United States;

    Chemical Engineering and Materials Science Department, Michigan State University, United States;

    Department of Physiology, Michigan State University, East Lansing, MI 48824, United States,Department of Radiology, Michigan State University, East Lansing, MI 48824, United States,Michigan State University, Departments of Physiology and Radiology, Biomedical Physical Science Bldg., 567 Wilson Road, East Lansing, MI 48824, United States;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Osteoblast; Microcracks; Attachment; Alignment; Maturation;

    机译:成骨细胞;微裂纹;附件;对准;成熟度;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号