Effects of genetic variants for the calpastatin gene on calpastatin activity and meat tenderness in Hanwoo (Korean cattle)

Hoyoung Chung; Michael Davis

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Effects of genetic variants for the calpastatin gene on calpastatin activity and meat tenderness in Hanwoo (Korean cattle)

机译：钙蛋白酶抑制剂基因的遗传变异对韩宇（韩国牛）中钙蛋白酶抑制剂活性和肉嫩度的影响

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摘要

This study was designed to investigate the effects of calpastatin genotypes determined by PCR-SSCP (polymerase chain reaction-single strand conformation polymorphism) on calpastatin activity (CAC) and Warner-Bratzler Shear Force (WBS). Longissimus muscles were prepared from 379 Hanwoo bulls aged approximately 20 months. The selection of PCR primers was based on exons (27 and 28) of the bovine calpastatin cDNA sequences, and genetic variants were detected by SSCP analysis using Taq I restriction enzymes. Sequencing analysis confirmed 4 restriction sites (nucleotide positions 52, 67, 796, and 1369), and a genetic variant was verified at a nucleotide position 641 (C/T substitutions) based on sequences (AF281256). The CAST28 genotypes showing allele frequencies of C (0.429) and T (0.571) were significantly associated with CAC and WBS. A significant positive residual correlation (r = 0.121, P = 0.02) between CAC and WBS was obtained.

机译：本研究旨在研究通过PCR-SSCP（聚合酶链反应-单链构象多态性）确定的钙蛋白酶抑制剂基因型对钙蛋白酶抑制剂活性（CAC）和Warner-Bratzler剪切力（WBS）的影响。从379名约20个月大的Hanwoo公牛制备了Longissimus肌肉。 PCR引物的选择基于牛钙抑素cDNA序列的外显子（27和28），并使用Taq I限制性内切酶通过SSCP分析检测了遗传变异。测序分析证实了4个限制性酶切位点（核苷酸位置52、67、796和1369），并根据序列（AF281256）在核苷酸位置641（C / T取代）处证实了遗传变异。显示C（0.429）和T（0.571）等位基因频率的CAST28基因型与CAC和WBS显着相关。在CAC和WBS之间获得了显着的正残留相关性（r = 0.121，P = 0.02）。

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来源
《Meat Science》 |2012年第3期|p.711-714|共4页
作者
Hoyoung Chung; Michael Davis;
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作者单位

Animal Genetic Improvement Division, National Institute of Animal Science, Seonghwan, Cheonan 333801, Republic of Korea,Bovine Functional Cenomics Laboratory ARS/USDA, 10300 Baltimore, Beltsville, MD 20705, USA;

Department of Animal Sciences, The Ohio State University, Columbus, OH 43210, USA;

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收录信息美国《科学引文索引》(SCI);
原文格式 PDF
正文语种 eng
中图分类
关键词
calpastatin; meat tenderness; genetic variants;

机译：钙抑素肉嫩;基因变异;

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机译：钙蛋白酶和μ钙蛋白酶的突变与韩牛（韩国牛）的肉嫩度，风味和多汁性有关：钙蛋白酶抑制素/μ钙蛋白酶复合物中取代作用的分子模型
2. micro -Calpain, calpastatin, and growth hormone receptor genetic effects on preweaning performance, carcass quality traits, and residual variance of tenderness in Angus cattle selected to increase minor haplotype and allele frequencies. [J] . Tait R. G. Jr., Shackelford S. D., Wheeler T. L., Journal of Animal Science . 2014,第2期

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3. μ-Calpain, calpastatin, and growth hormone receptor genetic effects on preweaning performance, carcass quality traits, and residual variance of tenderness in Angus cattle selected to increase minor haplotype and allele frequencies [J] . Bennett?G. L. Journal of animal science . 2014,第2期

机译：μ-钙蛋白酶，钙蛋白酶抑素和生长激素受体遗传因素对安格斯牛的断奶前性能，car体品质性状和嫩度残留变异的影响，以增加较小的单倍型和等位基因频率
4. ASSOCIATION OF SINGLE NUCLEOTIDE POLYMORPHISMS IN THE μ-CALPAIN AND CALPASTATIN GENE WITH PROTEASES ACTIVITIES IN RETINTA BEEF CATTLE: PRELIMINARY RESULTS [C] . Gai Zhiyi, rnGao Xiufang Proceedings of 53rd International Congress of Meat Science and Technology . 2007

机译：视网膜牛牛中μ钙蛋白和钙抑素基因中的单个核苷酸多态性与蛋白酶活性的关系：初步结果
5. Heritabilities and phenotypic and genetic correlations for bovine postrigor calpastatin activity and measures of meat tenderness and muscle growth. [D] . Shackelford, Steven Dean. 1992

机译：牛postrigor calpastatin活性的遗传力，表型和遗传相关性以及肉嫩度和肌肉生长的度量。
6. Genetic variants and signatures of selective sweep of Hanwoo population (Korean native cattle) [O] . Taeheon Lee, Seoae Cho, Kang Seok Seo, 2013

机译：Hanwoo种群（韩国本土牛）选择性扫描的遗传变异和特征
7. New SNP in calpastatin gene associated with meat tenderness and frequency in different cattle breeds: abstract [O] . Iguácel Quintana, Laura P., Calvo Lacosta, Jorge Hugo, Kirinus, J.K., 2013

机译：钙抑素基因中的新SNP与不同牛品种的肉嫩度和频率相关：摘要

Effects of genetic variants for the calpastatin gene on calpastatin activity and meat tenderness in Hanwoo (Korean cattle)

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