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首页> 外文期刊>Meat Science >Genotypic characterization of Brochothrix thermosphacta isolated during storage of minced pork under aerobic or modified atmosphere packaging conditions
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Genotypic characterization of Brochothrix thermosphacta isolated during storage of minced pork under aerobic or modified atmosphere packaging conditions

机译:有氧或改良气氛包装条件下切碎的猪肉储存过程中分离的嗜热布鲁氏菌的基因型鉴定

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摘要

A total of 306 colonies were isolated from the selective medium for Brochothrix spp., during the spoilage of minced pork stored at 0, 5, 10 and 15 ℃ and packed aerobically and under modified atmosphere packaging conditions (MAP). Brochothrix biodiversity was assessed by Pulsed Field Gel Electrophoresis (PFGE), and representative strains were further analysed by Rep-PCR using primer (GTG)_5 and Sau-PCR with primers SAG_(1-) and SAG_2. Although, different results were obtained from the different methods, a significant diversity among isolates recovered from aerobic conditions was observed. On the contrary, isolates from MAP showed a lower degree of heterogeneity. The storage conditions affected the Brochothrix diversity, the strains isolated in the initial stage being different from the ones present at the final stage of storage at chill temperatures. A representative number of isolates, based on the results of the clustering by molecular methods, were subjected to 16S rRNA gene sequencing, revealing that all belonged to Brochothrix thermosphacta.
机译:在储存于0、5、10和15℃的切碎的猪肉变质期间,从Brochothrix spp。的选择性培养基中分离出总共306个菌落,并在有氧条件下和在改良的大气包装条件下(MAP)进行包装。通过脉冲场凝胶电泳(PFGE)评估了Brochothrix的生物多样性,并通过使用引物(GTG)_5的Rep-PCR和使用引物SAG_(1-)和SAG_2的Sau-PCR进一步分析了代表性菌株。尽管从不同的方法获得了不同的结果,但在有氧条件下回收的分离株之间存在显着差异。相反,来自MAP的分离物显示出较低的异质性。储存条件影响了Brochothrix的多样性,在初始阶段分离的菌株与在低温下储存的最后阶段存在的菌株不同。基于分子方法的聚类结果,对代表性的分离株进行了16S rRNA基因测序,结果表明所有分离株均属于嗜热雄芽孢杆菌。

著录项

  • 来源
    《Meat Science》 |2012年第4期|p.735-738|共4页
  • 作者单位

    Laboratory of Microbiology and Biotechnology of Foods, Department of Food Science and Technology. Agricultural University of Athens, lera Odos 75, Athens 11855, Greece,National Agricultural Research Foundation, Institute of Technology of Agricultural Products, Sofokli Venizelou 1, Lycovrissi 14123, Greece;

    Laboratory of Microbiology and Biotechnology of Foods, Department of Food Science and Technology. Agricultural University of Athens, lera Odos 75, Athens 11855, Greece;

    DIVAPRA, Agricultural Microbiology and Food Technology Sector, Faculty of Agriculture, University of Turin, Italy;

    DIVAPRA, Agricultural Microbiology and Food Technology Sector, Faculty of Agriculture, University of Turin, Italy;

    Laboratory of Microbiology and Biotechnology of Foods, Department of Food Science and Technology. Agricultural University of Athens, lera Odos 75, Athens 11855, Greece;

  • 收录信息 美国《科学引文索引》(SCI);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    pork; brochothrix thermosphacta; PFCE; Rep-PCR; Sau-PCR;

    机译:猪肉;拟南芥PFCE;重复PCR Sau PCR;

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