Supplementation to the MRS medium for the cultivation of fastidious beer spoilage bacteria

A. Gillet; M.-H. Dupuche; N. Velings

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Supplementation to the MRS medium for the cultivation of fastidious beer spoilage bacteria

机译：补充MRS培养基以培养啤酒中的变质细菌

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The rapid detection and cultivation of the specific beer spoilage bacteria has always been a problem and still unresolved for some of the most fastidious Lactobacillus and Pediococcus species. This paper presents a new medium based on a adaptation of the MRS medium proposed by DeMan, Rogosa & Sharpe in 1960. The new medium is compared to others in term of size of the colonies on plates and kinetic of growth in liquid media. The kinetic is followed by optical density as well as cfu enumerations. In conclusion, we can say that with the new medium, we can easily cultivate and count some of the most fastidious bacterial beer spoilers.

机译：快速检测和培养特定的啤酒变质细菌一直是一个问题，但对于某些最挑剔的乳酸菌和Pecococcus菌种仍未解决。本文介绍了一种新的培养基，该培养基基于DeMan，Rogosa和Sharpe在1960年提出的MRS培养基的改编。在平板上菌落的大小和液体培养基中生长的动力学方面，将这种新培养基与其他培养基进行了比较。动力学之后是光密度以及cfu枚举。总而言之，我们可以说，使用新培养基，我们可以轻松培养和计数一些最严格的细菌啤酒扰流剂。

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《Monatsschrift fur Brauwissenschaft》 |2003年第2期|p.10-14|共5页
作者
A. Gillet; M.-H. Dupuche; N. Velings;
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Sopura SA, Rue de Trazegnies, 199, B-6180 Courcelles/Bekguym;

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正文语种 eng
中图分类酿造工业;
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1. Culture Medium for PCR-Based Analysis of Beer-Spoilage Bacteria [J] . Sabine Muller, Agnes Huber Brauwelt international . 2018,第4期

机译：基于PCR的啤酒腐败菌分析培养基
2. Development of detection medium for hard-to-culture beer-spoilage lactic acid bacteria [J] . Suzuki K, Asano S, Iijima K, Journal of applied microbiology . 2008,第5期

机译：难以培养的啤酒腐败乳酸菌检测培养基的开发
3. Study on ATP Production of Lactic Acid Bacteria in Beer and Development of a Rapid Pre-Screening Method for Beer-Spoilage Bacteria [J] . K. Suzuki, K. Iijima, K. Ozaki, Journal of the Institute of Brewing . 2005,第3期

机译：啤酒中乳酸菌ATP产生的研究及啤酒腐败菌快速预筛选方法的发展
4. Isolation of hop-sensitive variant from beer-spoilage Lactobacillus lindneri DSM 20692 and identification of genetic marker for differentiating beer spoilage ability of lactic acid bacteria [C] . European Brewery Convention congress . 2005

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5. Supplementation of various fatty acids enhances the growth rate of some anaerobic, cellulolytic bacteria in cellulose medium [D] . Daho, Amanda Elaine. 1999

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6. Isolation of a Hop-Sensitive Variant of Lactobacillus lindneri and Identification of Genetic Markers for Beer Spoilage Ability of Lactic Acid Bacteria [O] . Koji Suzuki, Kazumaru Iijima, Kazutaka Ozaki, 2005

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7. Supplementation to the MRSMedium for the Cultivation of Fastidious Beer Spoilage Bacteria. [O] . André GILLET, Marie-Hélène DUPUCHE, Nicolas VELINGS 2004

机译：对MRSMEDIUM进行补充，用于培养巨大的啤酒腐败细菌。
8. Evaluation of Medium Supplements for in Vitro Cultivation of Wuchereria bancrofti [R] . Franke, E. D., Riberu, W., Wiady, I. 1990

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Supplementation to the MRS medium for the cultivation of fastidious beer spoilage bacteria

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