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首页> 外文期刊>Journal of applied microbiology >Development of detection medium for hard-to-culture beer-spoilage lactic acid bacteria
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Development of detection medium for hard-to-culture beer-spoilage lactic acid bacteria

机译：难以培养的啤酒腐败乳酸菌检测培养基的开发

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Aims: To develop a detection medium for hard-to-culture beer-spoilage lactic acid bacteria (LAB). Methods and Results: Four hard-to-culture beer-spoilage strains of LAB, belonging to Lactobacillus paracollinoides and Lactobacillus lindneri, have been obtained by repeatedly subculturing the wild-type strains in beer. To develop a countermeasure against these hard-to-culture beer-spoilage LAB, a beer-based medium was modified. As a consequence, the supplementation of a small amount of de Man Rogosa Sharpe medium was found to enhance the growth of hard-to-culture beer-spoilage LAB strains obtained in this study. In addition, sodium acetate was shown to improve the selectivity of this beer-based medium. Further comparative study was performed with five other media widely used for the detection of beer-spoilage LAB in the brewing industry. This study revealed that the newly developed medium, designated advanced beer-spoiler detection (ABD) medium, possessed superior sensitivity for hard-to-culture beer-spoilage LAB and comparable sensitivity with easy-to-culture beer-spoilage LAB. Moreover, ABD medium was found to suppress the growth of nonspoilage micro-organisms, and thereby allow the selective growth of beer-spoilage LAB. Conclusions: Advanced beer-spoiler detection medium is considered as an effective tool for comprehensive detection of beer-spoilage LAB in breweries. Significance and Impact of the Study: The detection by ABD medium can be used as an indicator for differentiating the beer-spoilage ability of LAB without further confirmatory tests in breweries.
机译：目的：开发一种难以培养的啤酒变质乳酸菌（LAB）检测介质。方法和结果：通过反复在啤酒中对野生型菌株进行继代培养，获得了四种乳酸菌难以培养的乳酸菌菌株，分别属于副嗜酸乳杆菌和林德乳杆菌。为了制定针对这些难以培养的啤酒变质实验​​室的对策，对啤酒基培养基进行了改良。结果，发现补充少量的De Man Rogosa Sharpe培养基可增强在这项研究中获得的难以培养的啤酒变质LAB菌株的生长。另外，显示乙酸钠可改善这种啤酒基培养基的选择性。用广泛用于酿造行业中的啤酒变质LAB检测的其他五种介质进行了进一步的比较研究。这项研究表明，新开发的培养基，称为高级啤酒腐败物检测（ABD）培养基，具有对难以培养的啤酒腐败菌LAB的优异灵敏度，并且具有与易于培养的啤酒腐败菌LAB相当的灵敏度。此外，发现ABD培养基可抑制未腐败微生物的生长，从而使啤酒腐败LAB的选择性生长。结论：先进的啤酒腐败变质检测介质被认为是全面检测啤酒厂啤酒变质LAB的有效工具。研究的意义和影响：通过ABD培养基进行的检测可以用作区分LAB的啤酒腐败能力的指标，而无需在啤酒厂中进行进一步的验证性测试。

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