...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Nano communication networks >Cellular communication via directed protrusion growth: Critical length-scales and membrane morphology
【24h】

Cellular communication via directed protrusion growth: Critical length-scales and membrane morphology

机译：通过定向突起生长进行细胞通讯：关键的长度尺度和膜形态

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We investigated the growth of cell protrusions from adherent HEK 293 cells and their capability to bridge cytophobic Teflon~® AF microgaps. establishing a critical length scale, beyond which cells cannot probe free space. For this purpose, we employed a photolithography-based surface fabrication strategy for producing micropatterned substrates composed of glass and the amorphous polymer Teflon~® AF. Cell protrusions growing from HEK 293 cells on these substrates were confined to extend on 2 μm wide glass lanes, intersected by Teflon~® AF microgaps of various lengths between 2 and 16 μm. After 24 hours of incubation, the frequency of cell protrusions crossing the gap was found to be strongly dependent on the gap size. Gaps which are greater than 4 μm were found to be increasingly difficult to cross. Cell extensions crossing the microgaps either appeared as nanosized connections, in approximately 30% of all observed cases, or as microsized connections. Molecular transport in the established cell-to-cell connection across the micro-gap was investigated by activation of TRPM8 ion channels followed by supply of Ca~(2+) to one of the connected cells. The diffusion of the Ca~(2+) ions was visualized by means of a cell-permeant pre-fluorescent dye. We observed both open- and closed-ended intercellular connections in both nano- and microsized cell protrusions.
机译：我们研究了粘附的HEK 293细胞的细胞突起的生长及其弥合憎细胞Teflon®AF微间隙的能力。建立一个临界长度尺度，超过这个尺度，细胞将无法探测自由空间。为此，我们采用了基于光刻的表面制造策略来生产由玻璃和非晶聚合物AF组成的微图案化基板。从这些底物上的HEK 293细胞生长的细胞突起被限制在2μm宽的玻璃泳道上延伸，并与2至16μm之间各种长度的AF微间隙相交。温育24小时后，发现穿过间隙的细胞突起的频率强烈依赖于间隙的大小。发现大于4μm的间隙越来越难以穿过。跨越微间隙的细胞延伸物要么表现为纳米级连接，要么约占所有观察病例的30％，要么表现为微米级连接。通过激活TRPM8离子通道，然后向连接的细胞之一提供Ca〜（2+），研究了已建立的跨微间隙的细胞间连接中的分子运输。 Ca〜（2+）离子的扩散通过可透过细胞的预荧光染料观察到。我们在纳米和微型细胞突起中都观察到开放式和封闭式细胞间连接。

著录项

  • 来源
    《Nano communication networks》 |2015年第4期|178-182|共5页
  • 作者

    Haijiang Zhang; Anna Kim; Shijun Xu; Gavin D.M. Jeffries; Aldo Jesorka;

  • 作者单位

    Department of Chemical and Biological Engineering, Chalmers University of Technology, S-412-96 Goeteborg, Sweden;

    Department of Chemical and Biological Engineering, Chalmers University of Technology, S-412-96 Goeteborg, Sweden,Department of Physiology and Pharmacology, Karolinska Institutet, SE-17177 Stockholm, Sweden;

    Department of Chemical and Biological Engineering, Chalmers University of Technology, S-412-96 Goeteborg, Sweden;

    Department of Chemical and Biological Engineering, Chalmers University of Technology, S-412-96 Goeteborg, Sweden;

    Department of Chemical and Biological Engineering, Chalmers University of Technology, S-412-96 Goeteborg, Sweden;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Cell protrusions; Cell-to-cell connections; Teflon~® AF; Cell microgaps; Filopodia;

    机译：细胞突起;单元间连接;铁氟龙〜®AF;细胞微间隙;丝足纲;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号