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首页> 外文期刊>Nanobiotechnology, IET >Rapid synthesis of silver nanoparticles by Pseudomonas stutzeri isolated from textile soil under optimised conditions and evaluation of their antimicrobial and cytotoxicity properties
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Rapid synthesis of silver nanoparticles by Pseudomonas stutzeri isolated from textile soil under optimised conditions and evaluation of their antimicrobial and cytotoxicity properties

机译：在优化条件下从纺织土壤中分离的斯氏假单胞菌快速合成银纳米颗粒及其抗菌和细胞毒性

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摘要

Present study utilised textile soil isolated bacterium Pseudomonas stutzeri to synthesise extracellular silver nanoparticles (AgNPs) under optimised conditions. The synthesised AgNPs were characterised using ultraviolet-visible spectroscopy, Fourier transform infrared spectroscopy (FTIR) and transmission electron microscopy (TEM). Optimisation showed AgNPs synthesis within 8 h using 2mM Ag nitrate at pH9, temperature 80°C and maximum absorbance toward 400 nm. TEM analysis revealed spherical shape AgNPs and reduction in size upto 8 nm was observed under optimised conditions. FTIR spectra confirmed presence of proteins bound to AgNPs act as reducing agent. AgNPs showed strong antibacterial activity against multi-drug resistant (MDR) Escherichia coli and Klebsiella pneumoniae as demonstrated by disc diffusion and colony forming unit assays. Zone of inhibition increased with increasing concentration of AgNPs with maximum of 19 mm against E. coli and 17 mm against K. pneumoniae at concentration of 2 μg/disc. Furthermore, AgNPs did not show any cytotoxic effects on human epithelial cells as demonstrated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay even at 2 μg/ml concentration of AgNPs. The results of the present study suggest that AgNPs can be synthesised rapidly under optimised conditions and show strong antimicrobial property against MDR pathogens without having toxicity effect on human epithelial cells.
机译：本研究利用纺织土壤分离的细菌斯氏假单胞菌在优化条件下合成细胞外银纳米颗粒（AgNPs）。使用紫外可见光谱，傅立叶变换红外光谱（FTIR）和透射电子显微镜（TEM）对合成的AgNPs进行表征。优化显示，使用2mM硝酸银在pH9，温度80°C和最大吸光度向400 nm的情况下，在8小时内即可合成AgNPs。 TEM分析显示球形AgNPs，在优化条件下观察到尺寸减小了8 nm。 FTIR光谱证实与AgNPs结合的蛋白质的存在可作为还原剂。如盘扩散和菌落形成单位测定所示，AgNPs对多药耐药（MDR）大肠杆菌和肺炎克雷伯菌具有很强的抗菌活性。随着浓度的增加，AgNPs的抑制区域增加，针对大肠杆菌的最大浓度为19 mm，针对肺炎克雷伯菌的浓度最大为17 mm，而浓度为2μg/ disc。此外，AgNPs对人上皮细胞没有显示任何细胞毒性作用，如3-（4,5-二甲基噻唑-2-基）-2,5-二苯基溴化四氮唑测定所证明的，即使在2μg/ ml的AgNPs浓度下也是如此。本研究的结果表明，AgNPs可以在优化的条件下快速合成，并且显示出对MDR病原体的强大抗菌性能，而对人上皮细胞没有毒性作用。

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