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首页> 外文期刊>Nanobiotechnology, IET >In vitro effect of biogenic silver nanoparticles on sterilisation of tobacco leaf explants and for higher yield of protoplasts
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In vitro effect of biogenic silver nanoparticles on sterilisation of tobacco leaf explants and for higher yield of protoplasts

机译：生物纳米银纳米粒子对烟叶外植体杀菌和原生质体更高产量的体外作用

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摘要

Isolation of protoplasts from leaves is useful in plant research. The standard reference methods for isolation of protoplasts are tedious, cause cell damage, are low-yield, time consuming and prone to microbial contamination. To overcome this problem, the authors used silver nanoparticles (AgNPs) for the control of microbial contamination and with low concentration of enzyme mixture for rapid release of protoplasts. The leaf explants were sterilised with 95% ethanol for 30 s followed by biologically synthesised AgNPs (1, 5, 10 and 15 mg/l) for 10 to 20 min. The authors found that 10 mg/l concentration of AgNPs treatment on explants showed remarkable inhibitory effect on microbial contamination with high level of tolerance. Moreover, during protoplasts isolation, the addition of 10 mg/l AgNPs in leaf incubation buffer yielded 34% viable protoplasts in 3 h. This is the first report of AgNPs synthesis from waste plant medium, which was applied for the sterilisation of explants and rapid isolation of protoplasts.
机译：从叶片中分离原生质体可用于植物研究。分离原生质体的标准参考方法繁琐，引起细胞损伤，产量低，耗时且容易受到微生物污染。为了克服这个问题，作者使用银纳米颗粒（AgNPs）来控制微生物污染，并使用低浓度的酶混合物来快速释放原生质体。将叶片外植体用95％乙醇灭菌30 s，然后用生物合成的AgNP（1、5、10和15 mg / l）灭菌10至20分钟。作者发现，以10 mg / l的浓度对外植体进行AgNPs处理对微生物污染具有显着的抑制作用，并且具有较高的耐受性。此外，在原生质体分离过程中，在叶片温育缓冲液中添加10 mg / l AgNP可以在3小时内产生34％的可行原生质体。这是从废弃植物培养基中合成AgNPs的第一份报告，该报告被用于外植体的灭菌和原生质体的快速分离。

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