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The genome of Schmidtea mediterranea and the evolution of core cellular mechanisms

机译:Schmidtea Mediterraterranea的基因组及其核心细胞机制的演变

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摘要

The planarian Schmidtea mediterranea is an important model for stem cell research and regeneration, but adequate genome resources for this species have been lacking. Here we report a highly contiguous genome assembly of S. mediterranea, using long-read sequencing and a de novo assembler (MARVEL) enhanced for low-complexity reads. The S. mediterranea genome is highly polymorphic and repetitive, and harbours a novel class of giant retroelements. Furthermore, the genome assembly lacks a number of highly conserved genes, including critical components of the mitotic spindle assembly checkpoint, but planarians maintain checkpoint function. Our genome assembly provides a key model system resource that will be useful for studying regeneration and the evolutionary plasticity of core cell biological mechanisms.
机译:涡虫Schmidtea mediterranea是干细胞研究和再生的重要模型,但该物种缺乏足够的基因组资源。在这里,我们报告了地中海假单胞菌的高度连续的基因组装配,使用了长读测序和一个针对低复杂度读取增强的从头组装程序(MARVEL)。 Mediterraterranea基因组具有高度多态性和重复性,并具有一类新型的巨大逆转录元。此外,基因组装配缺少许多高度保守的基因,包括有丝分裂纺锤体装配检查点的关键成分,但涡虫保持了检查点的功能。我们的基因组组装提供了关键的模型系统资源,将对研究核心细胞生物学机制的再生和进化可塑性有用。

著录项

  • 来源
    《Nature》 |2018年第7690期|56-61|共6页
  • 作者单位

    Max Planck Inst Mol Cell Biol & Genet, Pfotenhauerstr 108, D-01307 Dresden, Germany;

    Heidelberg Inst Theoret Studies, Schloss Wolfsbrunnenweg 35, D-69118 Heidelberg, Germany;

    Max Planck Inst Mol Cell Biol & Genet, Pfotenhauerstr 108, D-01307 Dresden, Germany;

    Heidelberg Inst Theoret Studies, Schloss Wolfsbrunnenweg 35, D-69118 Heidelberg, Germany;

    Francis Crick Inst, 1 Midland Rd, London NW1 1AT, England;

    Max Planck Inst Mol Cell Biol & Genet, Pfotenhauerstr 108, D-01307 Dresden, Germany;

    Max Planck Inst Mol Cell Biol & Genet, Pfotenhauerstr 108, D-01307 Dresden, Germany;

    Max Planck Inst Mol Cell Biol & Genet, Pfotenhauerstr 108, D-01307 Dresden, Germany|Tech Univ Dresden, BIOTEC Ctr Regenerat Therapies Dresden, Deep Sequencing Grp, Cluster Excellence, Fetscherstr 105, D-01307 Dresden, Germany;

    Heidelberg Inst Theoret Studies, Schloss Wolfsbrunnenweg 35, D-69118 Heidelberg, Germany;

    Max Planck Inst Mol Cell Biol & Genet, Pfotenhauerstr 108, D-01307 Dresden, Germany|Max Planck Inst Phys Komplexer Syst, Nothnitzer Str 38, D-01187 Dresden, Germany;

    Max Planck Inst Mol Cell Biol & Genet, Pfotenhauerstr 108, D-01307 Dresden, Germany;

    Max Planck Inst Mol Cell Biol & Genet, Pfotenhauerstr 108, D-01307 Dresden, Germany;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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