SMN and symmetric arginine dimethylation of RNA polymerase II C-terminal domain control termination

Zhao Dorothy Yanling; Gish Gerald; Braunschweig Ulrich; Li Yue; Ni Zuyao; Schmitges Frank W.; Zhong Guoqing; Liu Ke; Li Weiguo; Moffat Jason; Vedadi Masoud; Min Jinrong; Pawson Tony J.; Blencowe Benjamin J.; Greenblatt Jack F.

首页> 外文期刊>Nature >SMN and symmetric arginine dimethylation of RNA polymerase II C-terminal domain control termination

【24h】

SMN and symmetric arginine dimethylation of RNA polymerase II C-terminal domain control termination

机译：SMN和对称精氨酸二甲基化的RNA聚合酶II C末端域控制终止

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

The carboxy-terminal domain (CTD) of the RNA polymerase II (RNAP II) subunit POLR2A is a platform for modifications specifying the recruitment of factors that regulate transcription, mRNA processing, and chromatin remodelling. Here we show that a CTD arginine residue (R1810 in human) that is conserved across vertebrates is symmetrically dimethylated (me2s). This R1810me2s modification requires protein arginine methyltransferase 5 (PRMT5) and recruits the Tudor domain of the survival of motor neuron (SMN, also known as GEMIN1) protein, which is mutated in spinal muscular atrophy. SMN interacts with senataxin, which is sometimes mutated in ataxia oculomotor apraxia type 2 and amyotrophic lateral sclerosis. Because POLR2A R1810me2s and SMN, like senataxin, are required for resolving RNA-DNA hybrids created by RNA polymerase II that form R-loops in transcription termination regions, we propose that R1810me2s, SMN, and senataxin are components of an R-loop resolution pathway. Defects in this pathway can influence transcription termination and may contribute to neurodegenerative disorders.

机译：RNA聚合酶II（RNAP II）亚基POLR2A的羧基末端结构域（CTD）是修饰平台，用于指定募集调节转录，mRNA加工和染色质重塑的因子。在这里，我们显示了在整个脊椎动物中保守的CTD精氨酸残基（在人类中为R1810）是对称的二甲基化（me2s）。这种R1810me2s修饰需要蛋白质精氨酸甲基转移酶5（PRMT5），并募集运动神经元（SMN，也称为GEMIN1）蛋白质存活的Tudor域，该蛋白质在脊髓性肌萎缩症中发生突变。 SMN与senataxin相互作用，而senataxin有时在共济失调动眼性运动失用2型和肌萎缩性侧索硬化症中发生突变。由于POLR2A R1810me2s和SMN像senataxin一样，是解决由RNA聚合酶II创建的在转录终止区域形成R环的RNA-DNA杂种所必需的，因此我们建议R1810me2s，SMN和senataxin是R环分解途径的组成部分。该途径中的缺陷可能影响转录终止，并可能导致神经退行性疾病。

著录项

来源
《Nature》 |2016年第7584期|48-53|共6页
作者
Zhao Dorothy Yanling; Gish Gerald; Braunschweig Ulrich; Li Yue; Ni Zuyao; Schmitges Frank W.; Zhong Guoqing; Liu Ke; Li Weiguo; Moffat Jason; Vedadi Masoud; Min Jinrong; Pawson Tony J.; Blencowe Benjamin J.; Greenblatt Jack F.;
展开▼
作者单位

Univ Toronto, Donnelly Ctr, Toronto, ON M5S 3E1, Canada|Mt Sinai Hosp, Lunenfeld Tanenbaum Res Inst, 600 Univ Ave, Toronto, ON M5G 1X5, Canada|Univ Toronto, Dept Mol Genet, 100 Coll St, Toronto, ON M5S 1A8, Canada;

Mt Sinai Hosp, Lunenfeld Tanenbaum Res Inst, 600 Univ Ave, Toronto, ON M5G 1X5, Canada;

Univ Toronto, Donnelly Ctr, Toronto, ON M5S 3E1, Canada;

Univ Toronto, Donnelly Ctr, Toronto, ON M5S 3E1, Canada|Univ Toronto, Dept Comp Sci, Toronto, ON M5S 3G4, Canada;

Univ Toronto, Donnelly Ctr, Toronto, ON M5S 3E1, Canada;

Univ Toronto, Donnelly Ctr, Toronto, ON M5S 3E1, Canada;

Univ Toronto, Donnelly Ctr, Toronto, ON M5S 3E1, Canada;

Univ Toronto, Struct Genom Consortium, Toronto, ON M5G 1L7, Canada;

Univ Toronto, Struct Genom Consortium, Toronto, ON M5G 1L7, Canada;

Univ Toronto, Donnelly Ctr, Toronto, ON M5S 3E1, Canada|Univ Toronto, Dept Mol Genet, 100 Coll St, Toronto, ON M5S 1A8, Canada;

Univ Toronto, Struct Genom Consortium, Toronto, ON M5G 1L7, Canada;

Univ Toronto, Struct Genom Consortium, Toronto, ON M5G 1L7, Canada;

Mt Sinai Hosp, Lunenfeld Tanenbaum Res Inst, 600 Univ Ave, Toronto, ON M5G 1X5, Canada|Univ Toronto, Dept Mol Genet, 100 Coll St, Toronto, ON M5S 1A8, Canada;

Univ Toronto, Donnelly Ctr, Toronto, ON M5S 3E1, Canada|Univ Toronto, Dept Mol Genet, 100 Coll St, Toronto, ON M5S 1A8, Canada;

Univ Toronto, Donnelly Ctr, Toronto, ON M5S 3E1, Canada|Univ Toronto, Dept Mol Genet, 100 Coll St, Toronto, ON M5S 1A8, Canada;

展开▼
收录信息美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类
关键词

相似文献

外文文献
中文文献
专利

1. Arginine Citrullination at the C-Terminal Domain Controls RNA Polymerase II Transcription [J] . Sharma Priyanka, Lioutas Antonios, Fernandez-Fuentes Narcis, Molecular cell . 2019,第1期

机译：C-末端结构域的精氨酸瓜氨酸对照RNA聚合酶II转录
2. The RNA Polymerase II C-terminal Domain-interacting Domain of Yeast Nrd1 Contributes to the Choice of Termination Pathway and Couples to RNA Processing by the Nuclear Exosome [J] . Dong-hyuk Heo, Inhea Yoo, Jiwon Kong, The Journal of biological chemistry . 2013,第51期

机译：酵母NRD1的RNA聚合酶II C-末端结构域相互作用结构域有助于选择终止途径，并通过核外出致其对RNA处理的选择
3. TFIIH-Associated Cdk7 Kinase Functions in Phosphorylation of C-Terminal Domain Ser7 Residues, Promoter-Proximal Pausing, and Termination by RNA Polymerase II [J] . Kira Glover-Cutter, Stéphane Larochelle, Benjamin Erickson, Molecular and Cellular Biology . 2009,第20期

机译：TFIIH相关的Cdk7激酶功能在C末端域Ser7残基的磷酸化，启动子近端暂停和RNA聚合酶II终止中。
4. The CrkRS/CDK12 kinase is activated by a novel isoform of Cyclin K and phosphorylates and the C-terminal domain of RNA Pol II [C] . Annie Moradian, S.-W. Grace Cheng, Michael Kuzyk, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2009

机译：CrKRS / CDK12激酶通过细胞周期蛋白K和磷酸化合物的新型同种型和RNA POL II的C末端结构域激活
5. Characterization of the Association of mRNA Export Factor Yra1 with the C-terminal Domain of RNA Polymerase II in vivo and in vitro [D] . MacKellar, April Lee 2011

机译：mRNA出口因子Yra1与RNA聚合酶II的C末端域在体内和体外的关联的表征。
6. The RNA Polymerase II C-terminal Domain-interacting Domain of Yeast Nrd1 Contributes to the Choice of Termination Pathway and Couples to RNA Processing by the Nuclear Exosome [O] . Dong-hyuk Heo, Inhea Yoo, Jiwon Kong, 2013

机译：酵母Nrd1的RNA聚合酶II C末端相互作用的域有助于终止途径的选择和核外泌体对RNA处理的耦合。
7. The RNA polymerase II C-terminal domain-interacting domain of yeast Nrd1 contributes to the choice of termination pathway and couples to RNA processing by the nuclear exosome [O] . Heo, D., Yoo, I., Kong, J., 2013

机译：酵母Nrd1的RNa聚合酶II C-末端结构域相互作用结构域有助于终止途径的选择，并通过核外体偶联RNa加工

SMN and symmetric arginine dimethylation of RNA polymerase II C-terminal domain control termination

摘要

著录项

相似文献

相关主题

期刊订阅