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Synthetic non-oxidative glycolysis enables complete carbon conservation

机译:合成的非氧化性糖酵解可实现完全碳保留

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摘要

Glycolysis, or its variations, is a fundamental metabolic pathway in life that functions in almost all organisms to decompose external or intracellular sugars. The pathway involves the partial oxidation and splitting of sugars to pyruvate, which in turn is decarboxylated to produce acetyl-coenzyme A (CoA) for various biosynthetic purposes. The decarboxylation of pyruvate loses a carbon equivalent, and limits the theoretical carbon yield to only two moles of twocarbon (C2) metabolites per mole of hexose. This native route is a major source of carbon loss in biorefining and microbial carbon metabolism. Here we design and construct a non-oxidative, cyclic pathway that allows the production of stoichiometric amounts of C2 metabolites from hexose, pentose and triose phosphates without carbon loss. We tested this pathway, termed non-oxidative glycolysis (NOG), in vitro and in vivo in Escherichia coli. NOG enables complete carbon conservation in sugar catabolism to acetyl-CoA, and can be used in conjunction with C0_2 fixation1 and other one-carbon (CI) assimilation pathways to achieve a 100% carbon yield to desirable fuels and chemicals.
机译:糖酵解或其变化是生命中的基本代谢途径,几乎在所有生物中都起作用,以分解外部或细胞内的糖。该途径涉及糖的部分氧化和分裂成丙酮酸,然后将其脱羧以产生用于各种生物合成目的的乙酰辅酶A(CoA)。丙酮酸的脱羧会损失碳当量,并将理论碳产率限制为每摩尔己糖仅两摩尔两种碳(C2)代谢物。这种天然途径是生物精制和微生物碳代谢中碳损失的主要来源。在这里,我们设计并构建了一种非氧化性的循环途径,该途径允许从己糖,戊糖和磷酸三糖生产化学计量的C2代谢物而无碳损失。我们在大肠杆菌中体外和体内测试了这种途径,称为非氧化糖酵解(NOG)。 NOG在糖分解代谢为乙酰辅酶A的过程中实现了完全的碳保留,可与CO_2固定1和其他一碳(CI)同化途径结合使用,以实现所需燃料和化学物质的100%碳产率。

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  • 来源
    《Nature》 |2013年第7473期|693-697|共5页
  • 作者单位

    Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, 420 Westwood Plaza, Los Angeles, California 90095, USA,Department of Bioengineering, University of California,Los Angeles, 420 Westwood Plaza, Los Angeles, California 90095, USA;

    Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, 420 Westwood Plaza, Los Angeles, California 90095, USA;

    Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, 420 Westwood Plaza, Los Angeles, California 90095, USA,Institute for Genomics and Proteomics, University of California, Los Angeles, 420 Westwood Plaza, Los Angeles, California 90095, USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
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