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Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes

机译:通过延时显微镜对人类基因组进行表型分析,揭示了细胞分裂基因

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摘要

Despite our rapidly growing knowledge about the human genome, we do not know all of the genes required for some of the most basic functions of life. To start to fill this gap we developed a high-throughput phenotypic screening platform combining potent gene silencing by RNA interference, time-lapse microscopy and computational image processing. We carried out a genome-wide phenotypic profiling of each of the ~21,000 human protein-coding genes by two-day live imaging of fluorescently labelled chromosomes. Phenotypes were scored quantitatively by computational image processing, which allowed us to identify hundreds of human genes involved in diverse biological functions including cell division, migration and survival. As part of the Mitocheck consortium, this study provides an in-depth analysis of cell division phenotypes and makes the entire high-content data set available as a resource to the community.
机译:尽管我们对人类基因组的了解迅速增长,但我们并不了解生命中某些最基本功能所需的所有基因。为了填补这一空白,我们开发了一种高通量表型筛选平台,该平台结合了通过RNA干扰,延时显微镜和计算图像处理产生的有效基因沉默。通过对荧光标记的染色体进行两天的实时成像,我们对〜21,000个人类蛋白质编码基因中的每一个进行了全基因组表型分析。通过计算图像处理对表型进行了定量评分,这使我们能够鉴定数百种涉及多种生物学功能(包括细胞分裂,迁移和存活)的人类基因。作为Mitocheck财团的一部分,这项研究提供了对细胞分裂表型的深入分析,并使整个高含量数据集可作为社区的资源。

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  • 来源
    《Nature》 |2010年第7289期|721-727|共7页
  • 作者单位

    MitoCheck Project Group, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, D-69117 Heidelberg, Germany;

    rnMitoCheck Project Group, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, D-69117 Heidelberg, Germany;

    rnWellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1HH, UK MitoCheck Project Group, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, D-69117 Heidelberg, Germany (J.-K.H.);

    rnMitoCheck Project Group, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, D-69117 Heidelberg, Germany;

    rnMitoCheck Project Group, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, D-69117 Heidelberg, Germany Cell Biology/Biophysics Units, Structural, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, D-69117 Heidelberg, Germany BIOQUANT Centre University Heidelberg, INF 267, D-69120 Heidelberg, Germany (H.E.);

    rnMitoCheck Project Group, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, D-69117 Heidelberg, Germany Cell Biology/Biophysics Units, Structural, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, D-69117 Heidelberg, Germany;

    rnMitoCheck Project Group, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, D-69117 Heidelberg, Germany 3-V Biosciences GmbH, Wagistrasse 27, 8952 Schlieren, Switzerland (P.R.);

    rnMax Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, D-01307 Dresden, Germany;

    rnMitoCheck Project Group, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, D-69117 Heidelberg, Germany Institute of Biochemistry, Swiss Federal Institute of Technology Zurich (ETHZ), Schafmattstrasse 18, CH-8093 Zurich, Switzerland (M.H.);

    rnMitoCheck Project Group, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, D-69117 Heidelberg, Germany Karlsruhe Institute of Technology KIT, Herrmann-von-Helmholtz Platz 1, D-76344 Eggenstein-Leopoldshafen, Germany (U.L.);

    rnCell Biology/Biophysics Units, Structural, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, D-69117 Heidelberg, Germany;

    rnLeica Microsystems CMS GmbH, Am Friedensplatz 3, D-68165 Mannheim, Germany;

    rnEuropean Bioinformatics Institute, European Molecular Biology Laboratory, Cambridge CB10 1SD, UK;

    rnDivision of Theoretical Bioinformatics, German Cancer Research Center, Im Neuenheimer Feld 267, D-69120 Heidelberg, Germany;

    rnGene Expression, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, D-69117 Heidelberg, Germany;

    rnComputational Biology Unit, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, D-69117 Heidelberg, Germany;

    rnInstitute of Biochemistry, Swiss Federal Institute of Technology Zurich (ETHZ), Schafmattstrasse 18, CH-8093 Zurich, Switzerland;

    rnCell Biology/Biophysics Units, Structural, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, D-69117 Heidelberg, Germany;

    rnInstitute of Biochemistry, Swiss Federal Institute of Technology Zurich (ETHZ), Schafmattstrasse 18, CH-8093 Zurich, Switzerland;

    rnComputational Biology Unit, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, D-69117 Heidelberg, Germany;

    rnDivision of Theoretical Bioinformatics, German Cancer Research Center, Im Neuenheimer Feld 267, D-69120 Heidelberg, Germany;

    rnEuropean Bioinformatics Institute, European Molecular Biology Laboratory, Cambridge CB10 1SD, UK;

    rnInstitute for Molecular Pathology, Dr Bohr Gasse 7, A-1030 Vienna, Austria;

    rnMax Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, D-01307 Dresden, Germany;

    rnWellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1HH, UK;

    rnCell Biology/Biophysics Units, Structural, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, D-69117 Heidelberg, Germany;

    rnGene Expression, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, D-69117 Heidelberg, Germany;

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