• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Nature >DNA nanomechanics allows direct digital detection of complementary DNA and microRNA targets
【24h】

DNA nanomechanics allows direct digital detection of complementary DNA and microRNA targets

机译：DNA纳米力学可以直接数字检测互补的DNA和microRNA靶标

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Techniques to detect and quantify DNA and RNA molecules in biological samples have had a central role in genomics research. Over the past decade, several techniques have been developed to improve detection performance and reduce the cost of genetic analysis. In particular, significant advances in label-free methods have been reported. Yet detection of DNA molecules at concentrations below the femtomolar level requires amplified detection schemes. Here we report a unique nanomechanical response of hybridized DNA and RNA molecules that serves as an intrinsic molecular label. Nanomechanical measurements on a microarray surface have sufficient background signal rejection to allow direct detection and counting of hybridized molecules. The digital response of the sensor provides a large dynamic range that is critical for gene expression profiling. We have measured differential expressions of microRNAs in tumour samples; such measurements have been shown to help discriminate between the tissue origins of metastatic tumours. Two hundred picograms of total RNA is found to be sufficient for this analysis. In addition, the limit of detection in pure samples is found to be one attomolar. These results suggest that nanomechanical read-out of microarrays promises attomolar-level sensitivity and large dynamic range for the analysis of gene expression, while eliminating biochemical manipulations, amplification and labelling.
机译：检测和定量生物样品中DNA和RNA分子的技术在基因组学研究中发挥了核心作用。在过去的十年中，已经开发了几种技术来提高检测性能并降低基因分析的成本。特别地，已经报道了无标签方法的重大进展。然而，检测浓度低于飞摩尔水平的DNA分子需要扩增的检测方案。在这里，我们报告杂交的DNA和RNA分子作为内在分子标记的独特的纳米力学响应。微阵列表面上的纳米力学测量具有足够的背景信号抑制能力，可直接检测和计数杂交分子。传感器的数字响应提供了很大的动态范围，这对于基因表达谱分析至关重要。我们已经测量了肿瘤样品中微小RNA的差异表达；已经显示出这样的测量有助于区分转移性肿瘤的组织起源。发现200皮克的总RNA足以进行此分析。此外，发现纯样品中的检出限为1摩尔。这些结果表明，微阵列的纳米机械读出有望在基因表达分析中达到摩尔水平的灵敏度和较大的动态范围，同时消除生物化学操作，扩增和标记。

著录项

  • 来源
    《Nature》 |2009年第7276期|1075-1078|共4页
  • 作者

    Sudhir Husale; Henrik H. J. Persson; Ozgur Sahin;

  • 作者单位

    The Rowland Institute at Harvard, Harvard University, Cambridge, Massachusetts 02142, USA;

    Stanford Genome Technology Center, 855 California Avenue, Palo Alto, California 94304, USA;

    The Rowland Institute at Harvard, Harvard University, Cambridge, Massachusetts 02142, USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号