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A type Ⅲ effector ADP-ribosylates RNA-binding proteins and quells plant immunity

机译：Ⅲ型效应子ADP-核糖基化RNA结合蛋白并抑制植物免疫

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The bacterial plant pathogen Pseudomonas syringae injects effector proteins into host cells through a type Ⅲ protein secretion system to cause disease. The enzymatic activities of most of P. syringae effectors and their targets remain obscure. Here we show that the type Ⅲ effector HopU1 is a mono-ADP-ribosyltransferase (ADP-RT). HopU1 suppresses plant innate immunity in a manner dependent on its ADP-RT active site. The HopU1 substrates in Arabidopsis thaliana extracts were RNA-binding proteins that possess RNA-recognition motifs (RRMs). A. thaliana knockout lines defective in the glycine-rich RNA-binding protein GRP7 (also known as AtGRP7), a HopUl substrate, were more susceptible than wild-type plants to P. syringae. The ADP-ribosylation of GRP7 by HopU1 required two arginines within the RRM, indicating that this modification may interfere with GRP7's ability to bind RNA. Our results suggest a pathogenic strategy where the ADP-ribosylation of RNA-binding proteins quells host immunity by affecting RNA metabolism and the plant defence transcriptome.
机译：细菌性植物病原体丁香假单胞菌通过Ⅲ型蛋白分泌系统将效应蛋白注入宿主细胞，从而引起疾病。大多数丁香假单胞菌效应子及其靶标的酶促活性仍然不清楚。在这里，我们显示Ⅲ型效应子HopU1是单ADP-核糖基转移酶（ADP-RT）。 HopU1依赖于其ADP-RT活性位点抑制植物的先天免疫。拟南芥提取物中的HopU1底物是具有RNA识别基序（RRM）的RNA结合蛋白。富含甘氨酸的RNA结合蛋白GRP7（也称为AtGRP7）（一种HopU1底物）中的拟南芥敲除品系比野生型植物对丁香假单胞菌更敏感。 HopU1对GRP7进行ADP核糖基化在RRM中需要两个精氨酸，这表明这种修饰可能会干扰GRP7结合RNA的能力。我们的结果提出了一种致病策略，其中RNA结合蛋白的ADP-核糖基化通过影响RNA代谢和植物防御转录组来抑制宿主免疫。

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