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首页> 外文期刊>Nature >Mitochondrial remnant organelles of Giardia function in iron-sulphur protein maturation
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Mitochondrial remnant organelles of Giardia function in iron-sulphur protein maturation

机译：贾第鞭毛虫的线粒体残余细胞器在铁硫蛋白成熟中的作用

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Giardia intestinalis (syn. lamblia) is one of the most widespread intestinal protozoan pathogens worldwide, causing hundreds of thousands of cases of diarrhoea each year. Giardia is a member of the diplomonads, often described as an ancient protist group whose primitive nature is suggested by the lack of typical eukaryotic organelles (for example, mitochondria, peroxisomes), the presence of a poorly developed endomembrane system and by their early branching in a number of gene phytogenies. The discovery of nuclear genes of putative mitochondrial ancestry in Giardia and the recent identification of mitochondrial remnant organelles in amitochondrial protists such as Entamoeba histolytica and Trachipleistophora hominis suggest that the eukaryotic amitochondrial state is not a primitive condition but is rather the result of reductive evolution. Using an in vitro protein reconstitution assay and specific antibodies against IscS and IscU―two mitochondrial marker proteins involved in iron-sulphur cluster biosynthesis―here we demonstrate that Giardia contains mitochondrial remnant organelles (mitosomes) bounded by double membranes that function in iron-sulphur protein maturation. Our results indicate that Giardia is not primitively amitochondrial and that it has retained a functional organelle derived from the original mitochondrial endosymbiont.
机译：贾第鞭毛虫（Syn。lamblia）是全世界最广泛的肠道原生动物病原体之一，每年引起数十万例腹泻。贾第虫（Giardia）是双胞胎的成员，通常被描述为一个古老的生物主义者群体，其原始本性是由于缺乏典型的真核细胞器（例如线粒体，过氧化物酶体），发育不完善的内膜系统以及它们在早期的分支而引起的。许多基因植物。在贾第鞭毛虫中发现了假定的线粒体祖先的核基因，并在线粒体原生质体如Entamoeba histolytica和Trachipleistophora hominis中线粒体残余细胞器的最新鉴定表明，真核线粒体状态不是原始条件，而是还原性进化的结果。使用体外蛋白质重建测定法和针对IscS和IscU的特异性抗体-参与铁硫簇生物合成的两种线粒体标记蛋白-在这里我们证明了贾第鞭毛虫含有线粒体残余细胞器（线粒体），其在铁硫蛋白中起着双重作用成熟。我们的结果表明，贾第鞭毛虫并不是原始的线粒体，并且保留了源自原始线粒体内共生体的功能性细胞器。

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