Two-photon microscopy with simultaneous standard and extended depth of ﬁeld using a tunable acoustic gradient-index lens

Nicolas OlivierAlexandre Mermillod-Blondin Craig B. Arnold and Emmanuel Beaurepaire

首页> 外文期刊>Optics Letters >Two-photon microscopy with simultaneous standard and extended depth of ﬁeld using a tunable acoustic gradient-index lens

【24h】

Two-photon microscopy with simultaneous standard and extended depth of ﬁeld using a tunable acoustic gradient-index lens

机译：双光子显微镜，使用可调声梯度折射率透镜，同时实现标准和扩展的场深

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

We describe a simple setup that allows depth of ﬁeld switching at kilohertz rates in a nonlinear microscope.nBeam proﬁle and/or divergence are modulated using a tunable, acoustically driven gradient-index ﬂuid lens.nWe demonstrate two modulation strategies, one based on fast varifocus scanning during each pixel and thenother based on pseudo-Bessel beam excitation. Average beam shape is switched every line during scanning,nresulting in the interlaced acquisition of two different images. We apply this approach to the simultaneousnstandard and 4.5u0001-extended depth-of-ﬁeld imaging of developing embryos. © 2009 Optical Society ofnAmerican.

机译：我们描述了一种简单的设置，可以在非线性显微镜中以千赫兹的速率进行深度切换。n使用可调谐的，声驱动的梯度折射率流体透镜来调制光束轮廓和/或发散。n我们展示了两种调制策略，一种基于快速变焦。在每个像素期间进行扫描，然后再基于伪贝塞尔光束激发进行扫描。在扫描过程中，每条线的平均光束形状会发生切换，从而导致隔行采集两个不同的图像。我们将此方法应用于正在发育的胚胎的同时标准和4.5u0001扩展的景深成像。 ©2009美国光学学会。

著录项

来源
《Optics Letters》 |2009年第11期|p.1684-1686|共3页
作者
Nicolas OlivierAlexandre Mermillod-Blondin Craig B. Arnold and Emmanuel Beaurepaire;
展开▼
作者单位

Laboratory for Optics and Biosciences, Ecole Polytechnique, CNRS, INSERM, F-91128 Palaiseau, France2Department of Mechanical and Aerospace Engineering, Princeton University, Princeton, New Jersey 08544, USA* Corresponding author: emmanuel.beaurepaire@polytechnique.edu;

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类
关键词

相似文献

外文文献
中文文献
专利

1. Two-photon microscopy with simultaneous standard and extended depth of field using a tunable acoustic gradient-index lens [J] . Nicolas Olivier, Alexandre Mermillod-Blondin, Craig B. Arnold, Optics Letters . 2009,第11期

机译：双光子显微镜，同时使用可调声梯度折射率透镜，同时具有标准和扩展景深
2. Resolution enhancement in an extended depth of field for volumetric two-photon microscopy [J] . Optics Letters . 2020,第11期

机译：体积二光子显微镜的延长景深的分辨率增强
3. Stimulated emission reduced fluorescence microscopy: a concept for extending the fundamental depth limit of two-photon fluorescence imaging [J] . Lu Wei, Zhixing Chen, Wei Min Biomedical Optics Express . 2012,第6期

机译：受激发射减少荧光显微镜：扩展双光子荧光成像的基本深度极限的概念
4. Ultrafast axial-scanning multifocus photoacoustic microscopy with extended depth-of-field based on a tunable acoustic gradient lens and fiber delay network [C] . Xianlin Song, Jianshuang Wei, Lingfang Song Conference on Diagnostic and Therapeutic Applications of Light in Cardiology . 2021

机译：超快轴向扫描多焦点光声显微镜，其基于可调声学梯度镜头和光纤延迟网络的延长景深
5. Acoustic metamaterials: Tunable gradient-index phononic crystals for acoustic wave manipulation [D] . Lin, Sz-Chin Steven 2012

机译：声学超材料：用于声波操纵的可调梯度折射率声子晶体
6. Stimulated emission reduced fluorescence microscopy: a concept for extending the fundamental depth limit of two-photon fluorescence imaging [O] . Lu Wei, Zhixing Chen, Wei Min 2012

机译：受激发射减少荧光显微镜：扩展双光子荧光成像的基本深度极限的概念
7. Two-photon microscopy with simultaneous standard and extended depth of field using a tunable acoustic gradient-index lens. [O] . Olivier Nicolas, Mermillod-Blondin Alexandre, Arnold Craig, 2009

机译：双光子显微镜，同时使用可调声梯度折射率透镜，同时具有标准和扩展景深。
8. Simultaneous One- and Two-Photon Processes in the Photodissociation of NCNO Using a Tunable Dye Laser [R] . Nadler, I., Pfab, J., Radhakrishnan, G., 1983

机译：使用可调谐染料激光在NCNO光解离中同时进行单光子和双光子过程

Two-photon microscopy with simultaneous standard and extended depth of ﬁeld using a tunable acoustic gradient-index lens

摘要

著录项

相似文献

相关主题

期刊订阅