Two-photon microscopy with simultaneous standard and extended depth of field using a tunable acoustic gradient-index lens

Nicolas Olivier; Alexandre Mermillod-Blondin; Craig B. Arnold; Emmanuel Beaurepaire

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Two-photon microscopy with simultaneous standard and extended depth of field using a tunable acoustic gradient-index lens

机译：双光子显微镜，同时使用可调声梯度折射率透镜，同时具有标准和扩展景深

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We describe a simple setup that allows depth of field switching at kilohertz rates in a nonlinear microscope. Beam profile and/or divergence are modulated using a tunable, acoustically driven gradient-index fluid lens. We demonstrate two modulation strategies, one based on fast varifocus scanning during each pixel and the other based on pseudo-Bessel beam excitation. Average beam shape is switched every line during scanning, resulting in the interlaced acquisition of two different images. We apply this approach to the simultaneous standard and 4.5X-extended depth-of-field imaging of developing embryos.

机译：我们描述了一种简单的设置，该设置允许在非线性显微镜中以千赫兹速率切换景深。光束轮廓和/或发散度使用可调的，声学驱动的梯度折射率流体透镜进行调制。我们演示了两种调制策略，一种基于每个像素期间的快速变焦扫描，另一种基于伪贝塞尔光束激励。在扫描过程中，每条线会切换平均光束形状，从而交错采集两个不同的图像。我们将这种方法应用于发育中的胚胎的同时标准和4.5倍扩展景深成像。

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《Optics Letters》 |2009年第11期|共3页
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Nicolas Olivier; Alexandre Mermillod-Blondin; Craig B. Arnold; Emmanuel Beaurepaire;
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1. Two-photon microscopy with simultaneous standard and extended depth of field using a tunable acoustic gradient-index lens [J] . Nicolas Olivier, Alexandre Mermillod-Blondin, Craig B. Arnold, Optics Letters . 2009,第11期

机译：双光子显微镜，同时使用可调声梯度折射率透镜，同时具有标准和扩展景深
2. Resolution enhancement in an extended depth of field for volumetric two-photon microscopy [J] . Optics Letters . 2020,第11期

机译：体积二光子显微镜的延长景深的分辨率增强
3. Extended depth-of-field 3D endoscopy with synthetic aperture integral imaging using an electrically tunable focal-length liquid-crystal lens [J] . Wang Yu-Jen, Shen Xin, Lin Yi-Hsin, Optics Letters . 2015,第15期

机译：使用电子可调焦距液晶透镜通过合成孔径积分成像实现扩展的景深3D内窥镜检查
4. Ultrafast axial-scanning multifocus photoacoustic microscopy with extended depth-of-field based on a tunable acoustic gradient lens and fiber delay network [C] . Xianlin Song, Jianshuang Wei, Lingfang Song Conference on Diagnostic and Therapeutic Applications of Light in Cardiology . 2021

机译：超快轴向扫描多焦点光声显微镜，其基于可调声学梯度镜头和光纤延迟网络的延长景深
5. Acoustic metamaterials: Tunable gradient-index phononic crystals for acoustic wave manipulation [D] . Lin, Sz-Chin Steven 2012

机译：声学超材料：用于声波操纵的可调梯度折射率声子晶体
6. Stimulated emission reduced fluorescence microscopy: a concept for extending the fundamental depth limit of two-photon fluorescence imaging [O] . Lu Wei, Zhixing Chen, Wei Min 2012

机译：受激发射减少荧光显微镜：扩展双光子荧光成像的基本深度极限的概念
7. Two-photon microscopy with simultaneous standard and extended depth of field using a tunable acoustic gradient-index lens. [O] . Olivier Nicolas, Mermillod-Blondin Alexandre, Arnold Craig, 2009

机译：双光子显微镜，同时使用可调声梯度折射率透镜，同时具有标准和扩展景深。
8. Imaging Using a Multifocal Aspheric Lens to Obtain Extended Depth of Field. [R] . George, N., Chi, W. 2005

机译：使用多焦点非球面透镜进行成像以获得扩展的景深。

Two-photon microscopy with simultaneous standard and extended depth of field using a tunable acoustic gradient-index lens

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