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Photodynamic effects on human periodontal-related cells in vitro

机译：体外对人牙周相关细胞的光动力效应

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摘要

Background: Photodynamic therapy (PDT) may be especially effective in combination with conventional periodontal therapy by its antimicrobial activities, but PDT may also exhibit other mechanisms that promote the healing of periodontal tissue. Therefore, the purpose of the present study was to evaluate the photodynamic effect of PDT on human periodontal ligament cells (hPDLCs) and human gingival fibroblasts (hFBs) in vitro and other possible mechanisms to promote periodontal healing. Methods: The proliferation of hPDLCs and hFBs was assessed by MTT assay. Cell attachment on cementum slices of hPDLCs and hFBs was evaluated by MTT assay. Type Ⅰ collagen synthesis of hPDLCs and hFBs was analyzed using enzyme linked immunosorbent assay. The alkaline phos-phatase (ALP) activity in hPDLCs was measured by p-nitrophenyl phosphate substrate reactions. Results: PDT treatment induced constant time-dependent growth of hPDLCs and hFBs at 24 h, 72 h and 6 days (P＜0.05). PDT treatment also promoted time-dependent hPDLCs and hFBs attachment on the cementum slices at 24 h, 72 h and 6 days compared to the controlled cells (P＜0.05). Type Ⅰ collagen synthesis of hPDLCs and hFBs was markedly stimulated by PDT in a time-dependent manner (P＜0.05). Likewise, a significant increase in the specific ALP activity in hPDLCs was observed (P＜0.05). Conclusions: The findings of this study indicate that PDT exhibited no cytotoxicity to hPDLCs or hFBs. Instead, it stimulated proliferation, attachment and collagen synthesis of hPDLCs and hFBs and ALP activity of hPDLCs. These effects might signal similar PDT activity on periodontal-related cells, and expanding the scope of its potential therapeutic utilization is very appealing.
机译：背景：光动力疗法（PDT）的抗菌活性可能与常规牙周疗法相结合特别有效，但PDT可能还表现出其他促进牙周组织愈合的机制。因此，本研究的目的是评估PDT在体外对人牙周膜细胞（hPDLC）和人牙龈成纤维细胞（hFBs）的光动力作用，以及其他可能促进牙周愈合的机制。方法：MTT法检测hPDLC和hFBs的增殖。通过MTT测定评估hPDLC和hFB的牙骨质切片上的细胞附着。用酶联免疫吸附法分析了hPDLC和hFBs的Ⅰ型胶原合成。 hPDLC中的碱性磷酸酶（ALP）活性是通过对硝基苯基磷酸酯底物反应来测量的。结果：PDT处理在24、72和6天时诱导hPDLC和hFB的持续时间依赖性生长（P ＜0.05）。与对照细胞相比，PDT处理在24、72和6天还促进了时间依赖性的hPDLC和hFBs附着在牙骨质切片上（P ＜0.05）。 PDT显着促进hPDLC和hFBs的Ⅰ型胶原合成，且呈时间依赖性（P ＜0.05）。同样，在hPDLCs中观察到比ALP活性显着增加（P ＜0.05）。结论：这项研究的结果表明PDT对hPDLC或hFB没有细胞毒性。相反，它刺激了hPDLC和hFB的增殖，附着和胶原合成，以及hPDLC的ALP活性。这些作用可能表明对牙周相关细胞具有类似的PDT活性，并且扩大其潜在治疗用途的范围非常有吸引力。

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  • 来源
    《Photodiagnosis and Photodynamic Therapy》 |2014年第3期|290-299|共10页
  • 作者

    Jing Qiao; Shiying Wang; Yi Wen; Huimei Jia;

  • 作者单位

    Department of Periodontology, Peking University School and Hospital of Stomatology, The First Outpatient Center, Xishenku Avenue 37, Xicheng District, Beijing 100034, China;

    Department of Periodontology, Peking University School and Hospital of Stomatology, The First Outpatient Center, Xishenku Avenue 37, Xicheng District, Beijing 100034, China;

    Department of Periodontology, Peking University School and Hospital of Stomatology, The First Outpatient Center, Xishenku Avenue 37, Xicheng District, Beijing 100034, China;

    Department of Periodontology, Peking University School and Hospital of Stomatology, The First Outpatient Center, Xishenku Avenue 37, Xicheng District, Beijing 100034, China;

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  • 正文语种 eng
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  • 关键词

    Photodynamic therapy; Human periodontal ligament cells; Human gingival fibroblasts; Periodontal therapy;

    机译：光动力疗法;人牙周膜细胞;人牙龈成纤维细胞;牙周治疗;

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