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首页> 外文期刊>Photodiagnosis and Photodynamic Therapy >Indocyanine green-based photodynamic therapy with 785 nm light emitting diode for oral squamous cancer cells
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Indocyanine green-based photodynamic therapy with 785 nm light emitting diode for oral squamous cancer cells

机译：基于吲哚菁绿的785 nm发光二极管光动力疗法治疗口腔鳞状细胞癌

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Background: The efficiency of photodynamic therapy (PDT) used in combination with Indocyanine green (ICG) and the light emitting diode (LED) on oral cancer was evaluated. The safety risk of ICG is known to be very low and ICG has a strong peak in the vicinity of 700-800 nm range which is thought to be a good candidate as a photosensitizer for PDT due to the deep penetration depth into the oral cancer tissue.Methods: The radiation intensity of homemade LED array was 50mW/cm~2 at 0.5 A. To evaluate the maximum efficiency of ICG-PDT on oral cancer, different wavelengths, ICG concentrations, irradiation interval times after administering ICG, and the time durations after PDT were tested. The cytotoxicity was determined by MTT assay, and apoptosis and necrosis were also observed by double staining with SYTO 16 green and PI.Results: The IC_(50) value was 10μM when 785 nm was irradiated, while it was very low in comparison with 630 nm and 895 nm. The values were not very different with varying interval time. The percentage of apoptotic cells increased gradually to 84% at 6h after 20 μm ICG-PDT and the percentage of necrotic cells dramatically increased to 65% at 3 h after 200 μm ICG-PDT. Conclusion: Using ICG-PDT with 785 nm LED light, the LED is regarded as a satisfying light source since cancer treatments in the oral region do not require focusing and increased depth of penetration due to longer wavelength enhances treatment effectiveness.
机译：背景：评价了光动力疗法（PDT）与吲哚菁绿（ICG）和发光二极管（LED）结合使用对口腔癌的效率。已知ICG的安全风险非常低，并且ICG在700-800 nm范围内有一个很强的峰，由于对口腔癌组织的深入渗透，它被认为是PDT的光敏剂的良好候选者方法：自制LED阵列在0.5 A时的辐射强度为50mW / cm〜2。评估ICG-PDT对口腔癌的最大功效，不同的波长，ICG浓度，施用ICG后的照射间隔时间以及持续时间经过PDT测试。 MTT法测定细胞毒性，SYTO 16 green和PI双染还观察到细胞凋亡和坏死。结果：照射785 nm时IC_（50）值为10μM，与630相比非常低。 nm和895 nm。这些值在间隔时间不同的情况下差别不大。 20μmICG-PDT后6 h，凋亡细胞的百分比逐渐增加至84％，而200μmICG-PDT后3 h，坏死细胞的百分比急剧增加至65％。结论：将ICG-PDT与785 nm LED光线配合使用，LED被认为是令人满意的光源，因为口腔区域的癌症治疗不需要聚焦，并且由于更长的波长而增加了穿透深度，从而提高了治疗效果。

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