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首页> 外文期刊>Photodiagnosis and Photodynamic Therapy >Continuous or fractionated photodynamic therapy? Comparison of three PDT schemes for ovarian peritoneal micrometastasis treatment in a rat model
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Continuous or fractionated photodynamic therapy? Comparison of three PDT schemes for ovarian peritoneal micrometastasis treatment in a rat model

机译：连续或分段光动力疗法？三种PDT方案在大鼠模型中治疗卵巢腹膜微转移的比较

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摘要

Objective: This experimental study aimed to compare three illumination schemes to optimize hexaminolaevulinate (HAL)-PDT in a rat tumor model with advanced ovarian cancer. Materials and methods: Peritoneal carcinomatosis was induced by intraperitoneal 5 x 10~6 NuTu-19 cells injection in 60 female rats Fisher 344. Carcinomatosis was obtained 50 days post-tumor induction. Four hours post-intraperitoneal HAL (Photocure ASA, Oslo, Norway) injection, three different schemes of PDT were performed during 25 min on a 1 cm2 area. (A) Fractionated illumination (n = 20) with an on-off cycle ("on": 2min and "off": 1 min) at 30mWcm~(-2) until a fluence of 30Jcm~(-2), (B) continuous illumination (n = 20) at 30mWcm~(-2) with a fluence of (45 Jcm~(-2)C) continuous illumination (n = 20) at 20mWcm~(-2) with a fluence of 30Jcm~(-2). Laser light was generated using a 532 nm KTP laser (Laser Quantum, Stockport, UK). Biopsies were taken 24 h after treatment. Quantitative histology was performed. Necrosis value was determined: 0-no necrosis to 4-full necrosis. Depth of necrosis was then measured for each sample and correlated to Necrosis value. Results: HAL-PDT was efficient in producing necrosis irrespective of the scheme. Tumor destruction was superior with fractionated illumination compared to both continuous illumination schemes regarding to the depth of necrosis (213±113 μm vs 154±133μm vs 171 ±155μm) (p<0.05) or to the full necrosis rate (50% vs 30% vs 10%) (p<0.0001). Conclusion: Fractionated illumination during photodynamic therapy (PDT) was shown to improve tumor response. Fractionated illumination with short intervals should be considered for an effective PDT of advanced ovarian cancer.
机译：目的：本实验研究旨在比较三种照明方案以优化晚期卵巢癌大鼠肿瘤模型中的六氨基戊酸（HAL）-PDT。材料与方法：通过腹膜内注射60只雌性Fisher 344大鼠腹腔5 x 10〜6 NuTu-19细胞诱导腹膜癌变。癌变在诱导后50天获得。腹腔注射HAL（Photocure ASA，奥斯陆，挪威）后四小时，在25分钟内于1 cm2面积上进行了三种不同的PDT方案。 （A）以30mWcm〜（-2）的开关周期（“ on”：2min and“ off”：1 min）进行分次照明（n = 20），直到通量为30Jcm〜（-2），（B ）在30mWcm〜（-2）下以（45 Jcm〜（-2）C的通量连续照射（n = 20））在20mWcm〜（-2）下以30Jcm〜（In的通量）连续照明（n = 20） -2）。使用532 nm KTP激光器（Laser Quantum，Stockport，英国）产生激光。治疗后24小时进行活检。进行定量组织学。确定坏死值：0-无坏死至4-完全坏死。然后测量每个样品的坏死深度，并将其与坏死值相关。结果：无论采用哪种方案，HAL-PDT均能有效产生坏死。与坏死深度（213±113μmvs 154±133μmvs 171±155μm）（p <0.05）或完全坏死率（50％vs 30％）相比，连续照明方案的肿瘤破坏优于连续照明方案vs 10％）（p <0.0001）。结论：在光动力疗法（PDT）期间局部照明可改善肿瘤反应。对于晚期卵巢癌的有效PDT，应考虑短间隔的分次照射。

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  • 来源
    《Photodiagnosis and Photodynamic Therapy》 |2010年第4期|p.251-257|共7页
  • 作者

    Juan Pablo Estevez; Manuel Ascencio; Pierre Colin; Marie Odile Farine; Pierre Collinet; SergeMordon;

  • 作者单位

    INSERM, U 703 - Univ. de Lille Nord de France - Lille University Hospital - CHRU, Lille, France,Department of Gynaecology and Obstetrics, Lille University Hospital - CHRU, Lille, France;

    INSERM, U 703 - Univ. de Lille Nord de France - Lille University Hospital - CHRU, Lille, France,Department of Gynaecology and Obstetrics, Lille University Hospital - CHRU, Lille, France;

    INSERM, U 703 - Univ. de Lille Nord de France - Lille University Hospital - CHRU, Lille, France;

    Department of Anatomo-pathology, Lille University Hospital - CHRU, Lille, France;

    INSERM, U 703 - Univ. de Lille Nord de France - Lille University Hospital - CHRU, Lille, France,Department of Gynaecology and Obstetrics, Lille University Hospital - CHRU, Lille, France;

    INSERM, U 703 - Univ. de Lille Nord de France - Lille University Hospital - CHRU, Lille, France,INSERM-U 703, 152, rue du Dr. Yersin, 59120 Loos, France;

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  • 正文语种 eng
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  • 关键词

    photodynamic therapy; light fractionatiort; ovarian cancer;

    机译：光动力疗法;轻馏分卵巢癌;

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