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In vitro efficiency and mechanistic role of indocyanine green as photodynamic therapy agent for human melanoma

机译：吲哚菁绿作为人黑素瘤光动力治疗剂的体外功效及机理

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Background: Photodynamic therapy (PDT) is a promising treatment for superficial cancer. However, poor therapeutic results have been reported for melanoma, due to the high melanin content. Indocyanine green (ICG) has near infrared absorption (700-800 nm) and melanins do not absorb strongly in this area. This study explores the efficiency of ICG as a PDT agent for human melanoma, and its mechanistic role in the cell death pathway.rnMethods: Human skin melanoma cells (Sk-Mel-28) were incubated with ICG and exposed to a low power Ti:Sapphire laser. Synchrotron-assisted Fourier transform infrared microspectroscopy and hierarchical cluster analysis were used to assess the cell damage and changes in lipid, protein, and nucleic acids. The cell death pathway was determined by analysis of cell viability and apoptosis and necrosis markers.rnResults: In the cell death pathway, ~1O_2 generation evoked rapid multiple consequences that trigger apoptosis after laser exposure for only 15min including the release of cytochrome c, the activation of total caspases, caspase-3, and caspase-9, the inhibition of NF-kB P65, and the enhancement of DNA fragmentation, and histone acetylation.rnConclusion: ICG/PDT can efficiently and rapidly induce apoptosis in human melanoma cells and it can be considered as a new therapeutic approach for topical treatment of melanoma.
机译：背景：光动力疗法（PDT）是一种用于浅表癌的有前途的疗法。然而，由于黑色素含量高，已经报道了黑色素瘤的治疗效果差。吲哚菁绿（ICG）具有近红外吸收（700-800 nm），黑色素在该区域吸收不强。本研究探讨了ICG作为PDT药物治疗人黑色素瘤的效率及其在细胞死亡途径中的作用。方法：将人皮肤黑色素瘤细胞（Sk-Mel-28）与ICG一起孵育并暴露于低功率Ti中：蓝宝石激光。同步加速器辅助的傅立叶变换红外显微技术和层次聚类分析用于评估细胞损伤以及脂质，蛋白质和核酸的变化。结果：在细胞死亡途径中，〜1O_2的产生引起快速的多重后果，激光照射仅15分钟后就触发了细胞凋亡，包括细胞色素c的释放，活化。半胱氨酸蛋白酶，半胱天冬酶3和半胱天冬酶9的表达，NF-κBP65的抑制，DNA片段化的增强和组蛋白乙酰化。被认为是局部治疗黑色素瘤的新治疗方法。

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