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首页> 外文期刊>Photodiagnosis and photodynamics therapy >Topical ALA—PDT modifies neutrophils' chemiluminescence, lymphocytes' interleukin-1beta secretion and serum level of transforming growth factor beta 1 in patients with nonmelanoma skin malignancies A clinical study
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Topical ALA—PDT modifies neutrophils' chemiluminescence, lymphocytes' interleukin-1beta secretion and serum level of transforming growth factor beta 1 in patients with nonmelanoma skin malignancies A clinical study

机译：局部ALA-PDT改变非黑色素瘤皮肤恶性肿瘤患者中性粒细胞的化学发光，淋巴细胞白介素1beta分泌和血清转化生长因子β1的水平临床研究

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Background: Photodynamic therapy (PDT) has been recognized as a noninvasive therapeutic approach for the effective treatment of tumors. It has been shown in studies conducted on malignant cell lines and various animal tumor models, that the interaction of photosensitizing substances with light leads to the release of cytotoxic substances and stimulates the immune response. Purpose: The aim of our study was to analyze the immune system response in patients undergoing photodynamic therapy due to basal cell carcinoma (BCC). Methods: Patients with skin malignancies have been treated by 10% delta-aminolevulinic acid (ALA) (Medac GmbH, Wedel, Germany) topically and light from a diode laser. Blood samples were obtained from each patient twice in the same day: before and 4h after photodynamic treatment procedure. In patients' serum the concentration of transforming growth factor beta1 (TGF-β1) was determined. Additionally the study has been conducted on lymphocytes and granulocytes from peripheral blood. In cell culture supernatants the concentration of interleukin 1beta (IL-1β), interleukin 2 (IL-2), interleukin 6 (IL-6), tumor necrosis factor alpha (TNFα), the percentile composition of patients' lymphocytes and the chemiluminescence of neutrophils have been measured. Results: We have observed a significant increase (p = 0.015) in the intensity of the neutrophil chemiluminescence and significant diminution (p = 0.006) of IL-1β concentration in supernatants. Similarly the serum level of TGF-β1 has been significantly decreased (p < 0.001). Conclusion: It is very likely that human immune system activity is modified by topical ALA—PDT and may potentially contribute to its final outcome.
机译：背景：光动力疗法（PDT）被公认为有效治疗肿瘤的一种非侵入性治疗方法。对恶性细胞系和各种动物肿瘤模型进行的研究表明，光敏物质与光的相互作用会导致细胞毒性物质的释放并刺激免疫反应。目的：我们的研究目的是分析由于基底细胞癌（BCC）而接受光动力治疗的患者的免疫系统反应。方法：皮肤恶性肿瘤患者已接受局部10％δ-氨基乙酰丙酸（ALA）（Medac GmbH，Wedel，Germany）和二极管激光的治疗。在同一天两次（分别在光动力治疗程序之前和之后4h）从每个患者中获取血液样本。测定患者血清中转化生长因子β1（TGF-β1）的浓度。另外，已经对来自外周血的淋巴细胞和粒细胞进行了研究。在细胞培养上清液中，白细胞介素1β（IL-1β），白细胞介素2（IL-2），白细胞介素6（IL-6），肿瘤坏死因子α（TNFα）的浓度，患者淋巴细胞的百分组成和化学发光中性粒细胞已被测量。结果：我们观察到中性粒细胞化学发光强度显着增加（p = 0.015），上清液中IL-1β浓度显着减小（p = 0.006）。同样，TGF-β1的血清水平也明显降低（p <0.001）。结论：局部ALA-PDT可能会改变人类免疫系统的活性，并可能有助于其最终结果。

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