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Enhancement of photodynamic inactivation of planktonic cultures of Staphylococcus aureus by DMMB-AuNPs

机译：DMMB-AUNPS的增强DMMB-AUNPS葡萄球菌氏菌培养物的灭活

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摘要

Photodynamic inactivation is a promising method for the treatment of infectious diseases. Nanotechnology through gold nanoparticles, as a tool to improve the delivery of photosensitizer is an attractive approach to enhance photodynamic inactivation of bacteria. Moreover, gold nanoparticles enchance the absorption of light due to their plasmon resonance. The aim of this study was to evaluate in vitro photodynamic inactivation effects of 1.9-Dimethyl-Methylene Blue (DMMB)-AuNPs associated with the red LED (lambda 630 eta m +/- 20 eta m, 125 mW, 12 J / cm(2), 192 s) on S. aureus strain. Eight experimental groups were studied: Control, LED, AuNPs, AuNPs + LED, DMMB, DMMB + LED, DMMB + AuNPs, DMMB + AuNPs + LED. After incubation, the number of bacteria surviving each treatment was determined and then enumerated by viable counting (CFU / mL). The logarithm of CFU / mL (CFU/mL log(10)) was calculated. All experiments realized in triplicate. The statistical analyses included one-way ANOVA tests, Tukey's multiple comparisons and nonlinear regression, p values 0.05 were considered statistically significant. According to results, the photodynamic inactivation of S. aureus on groups DMMB + LED and DMMB-AuNPs + LED, showed a significant reduction of the microbial load (p 0.0001) when compared to the Control group. The decimal reduction (RD) of these groups were 99.96 % (RD = 3) and 99.994 % (RD = 4) respectively. In conclusion, these findings demonstrated that photodynamic inactivation is enhanced by using DMMB-AuNPs on S. aureus.
机译：光动力灭活是一种治疗传染病的有希望的方法。纳米技术通过金纳米颗粒，作为改善光敏剂的递送的工具是一种有吸引力的方法，可以增强细菌的光动力灭活。此外，金纳米颗粒引起了由于其等离子体共振引起的光吸收。本研究的目的是评估与红色LED相关的1.9-二甲基 - 亚甲基蓝（DMMB）-AUNP的体外光动力灭活作用（Lambda 630 eta m +/-20η，125mW，12J / cm（ 2），192秒）对金黄色葡萄球菌菌株。研究了八个实验组：控制，LED，AUNP，AUNP + LED，DMMB，DMMB + LED，DMMB + AUNP，DMMB + AUNPS + LED。孵育后，测定每处理每种处理的细菌的数量，然后通过可行的计数（CFU / mL）来列举。计算CFU / mL（CFU / mL log（10））的对数。所有一式三份实现的实验。统计分析包括单向ANOVA测试，Tukey的多重比较和非线性回归，P值<0.05被认为是统计学意义。根据结果​​，与对照组相比，DMMB + LED和DMMB-AUNPS + LED对DMMB + LED和DMMB-AUNPS + LED的光动力动力灭活。这些基团的十进制减少（RD）分别为99.96％（RD = 3）和99.994％（RD = 4）。总之，这些研究结果表明，通过在金黄色葡萄球菌上使用DMMB-AUNP来增强光动力灭活。

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  • 来源
    《Photodiagnosis and Photodynamic Therapy》 |2020年第9期|101930.1-101930.7|共7页
  • 作者

    Monteiro Juliana S. C.; Rangel Emilia E.; de Oliveira Susana C. P. S.; Crugeira Pedro J. L.; Nunes Iago P. F.; Fagnani Sandra R. C. de A.; Sampaio Fernando J. P.; de Almeida Paulo F.; Pinheiro Antonio L. B.;

  • 作者单位

    Feira de Santana State Univ Dept Biol BR-44036900 Feira De Santa BA Brazil|Univ Fed Bahia Ctr Biophoton 62 Araujo Pinho Ave BR-40110150 Salvador BA Brazil;

    Feira de Santana State Univ Dept Biol BR-44036900 Feira De Santa BA Brazil;

    Univ Fed Bahia Ctr Biophoton 62 Araujo Pinho Ave BR-40110150 Salvador BA Brazil;

    Univ Fed Bahia Ctr Biophoton 62 Araujo Pinho Ave BR-40110150 Salvador BA Brazil|Univ Fed Bahia Inst Hlth Sci Lab Biotechnol & Ecol Microorganisms Reitor Miguel Calmon Ave S-N BR-40110100 Salvador BA Brazil;

    Feira de Santana State Univ Dept Biol BR-44036900 Feira De Santa BA Brazil|Univ Fed Bahia Ctr Biophoton 62 Araujo Pinho Ave BR-40110150 Salvador BA Brazil;

    Feira de Santana State Univ Dept Biol BR-44036900 Feira De Santa BA Brazil|Univ Fed Bahia Ctr Biophoton 62 Araujo Pinho Ave BR-40110150 Salvador BA Brazil;

    Feira de Santana State Univ Dept Biol BR-44036900 Feira De Santa BA Brazil|Univ Fed Bahia Ctr Biophoton 62 Araujo Pinho Ave BR-40110150 Salvador BA Brazil;

    Univ Fed Bahia Inst Hlth Sci Lab Biotechnol & Ecol Microorganisms Reitor Miguel Calmon Ave S-N BR-40110100 Salvador BA Brazil;

    Univ Fed Bahia Ctr Biophoton 62 Araujo Pinho Ave BR-40110150 Salvador BA Brazil|Natl Inst Basic Opt & Appl Life Sci 400 Trabalhador Sao Carlense Ave BR-13566590 Sao Carlos SP Brazil;

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  • 正文语种 eng
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  • 关键词

    Nanotechnology; Staphylococcus aureus; Antimicrobial photodynamic therapy; Gold nanoparticles; DMMB;

    机译：纳米技术;金黄色葡萄球菌;抗微生物光学性疗法;金纳米颗粒;DMMB;

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