• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Photodiagnosis and Photodynamic Therapy >Vaterite submicron particles designed for photodynamic therapy in cells
【24h】

Vaterite submicron particles designed for photodynamic therapy in cells

机译：Vaterite亚微米粒子设计用于电池的光动力学疗法

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background: Calcium carbonate (CaCO3) is one of the most abundant materials in the world. It has several different crystalline phases as present in the minerals: calcite, aragonite and vaterite, which are anhydrous crystalline polymorphs. Regarding the preparation of these microparticles, the most important aspect is the control of the polymorphism, particle size and material morphology. This study aimed to develop porous microparticles of calcium carbonate in the vaterite phase for the encapsulation of chloro-aluminum phthalocyanine (ClAlPc) as a photosensitizer (PS) for application in Photodynamic Therapy (TED).Methods: In this study, spherical vaterite composed of microparticles are synthesized by precipitation route assisted by polycarboxylate superplasticizer (PSS). The calcium carbonate was prepared by reacting a mixed solution of Na2CO3 with a CaCl2 solution at an ambient temperature, 25 degrees C, in the presence of polycarboxylate superplasticizer as a stabilizer. The photosensitizer was incorporated by adsorption technique in the CaCO3 microparticles. The CaCO3 microparticles were studied by scanning electron microscopy, steady-state, and their biological activity was evaluated using in vitro cancer cell lines by trypan blue exclusion method. The intracellular localization of ClAlPc was examined by confocal microscopy.Results: The CaCO3 microparticles obtained are uniform and homogeneously sized, non-aggregated, and highly porous microparticles. The calcium carbonate microparticles show an average size of 3 mu m average pore size of about 30-40 nm. The phthalocyanine derivative loaded-microparticles maintained their photophysical behavior after encapsulation. The captured carriers have provided dye localization inside cells. The in vitro experiments with ClAlPc-loaded CaCO3 microparticles showed that the system is not cytotoxic in darkness, but exhibits a substantial phototoxicity at 3 mu mol.L-1 of photosensitizer concentration and 10 J.cm(2) of light. These conditions are sufficient to kill about 80 % of the cells.Conclusions: All the performed physical-chemical, photophysical, and photobiological measurements indicated that the phthalocyanine-loaded CaCO3 microparticles are a promising drug delivery system for photodynamic therapy and photoprocesses.
机译：背景：碳酸钙（CaCO3）是世界上最丰富的材料之一。它在矿物质中存在几种不同的结晶阶段：方解石，金属石和Vaterite，其是无水晶体多晶型物。关于这些微粒的制备，最重要的方面是对多态性，粒度和材料形态的控制。该研究旨在在Vaterite期中开发多孔微粒，用于在光电疗法（TED）中涂抹氯铝酞菁（CLALPC）作为光敏剂（PS）。方法：在本研究中，球形Vaterite由通过聚羧酸盐超塑料（PSS）辅助的沉淀路径合成微粒。通过将Na 2 CO 3的混合溶液与CaCl 2溶液在环境温度，25℃下，在聚羧酸盐超塑化剂作为稳定剂存在下，制备碳酸钙。光敏化剂通过CaCO 3微粒中的吸附技术掺入。通过扫描电子显微镜，稳态来研究CaCO 3微粒，通过通过台式蓝色排除方法使用体外癌细胞系评估其生物活性。 ClalPC的细胞内定位通过共聚焦显微镜检查。结果：得到的CaCO 3微粒是均匀的，均匀的，非聚集的，高度多孔的微粒。碳酸钙微粒显示平均孔径为约30-40nm的平均尺寸。酞菁衍生物载体的微粒在包封后保持其光学性行为。捕获的载体在细胞内提供了染料定位。加载Clalpc的CaCO 3微粒的体外实验表明，该系统在黑暗中不是细胞毒性，但在3μmol.L-1的光敏剂浓度和10J.CM（2）光中表现出大量的光炎。这些条件足以杀死约80％的细胞。结论：所有所做的物理化学，光药和光生物测量表明，酞菁加载的CaCO 3微粒是用于光动力治疗和光处理的有希望的药物递送系统。

著录项

  • 来源
    《Photodiagnosis and Photodynamic Therapy》 |2020年第9期|101913.1-101913.7|共7页
  • 作者

    Souza Eliane F.; Ambrosio Jessica A. R.; Pinto Bruna C. S.; Beltrame Milton; Sakane Kumiko K.; Pinto Juliana G.; Ferreira-Strixino Juliana; Goncalves Erika P.; Simioni Andreza R.;

  • 作者单位

    Vale Paraiba Univ Res & Dev Inst IPD UNIVAP Av Shishima Hifumi 2911 BR-12244000 Sao Jose Dos Campos SP Brazil;

    Vale Paraiba Univ Res & Dev Inst IPD UNIVAP Av Shishima Hifumi 2911 BR-12244000 Sao Jose Dos Campos SP Brazil;

    Vale Paraiba Univ Res & Dev Inst IPD UNIVAP Av Shishima Hifumi 2911 BR-12244000 Sao Jose Dos Campos SP Brazil;

    Vale Paraiba Univ Res & Dev Inst IPD UNIVAP Av Shishima Hifumi 2911 BR-12244000 Sao Jose Dos Campos SP Brazil;

    Vale Paraiba Univ Res & Dev Inst IPD UNIVAP Av Shishima Hifumi 2911 BR-12244000 Sao Jose Dos Campos SP Brazil;

    Vale Paraiba Univ Res & Dev Inst IPD UNIVAP Av Shishima Hifumi 2911 BR-12244000 Sao Jose Dos Campos SP Brazil;

    Vale Paraiba Univ Res & Dev Inst IPD UNIVAP Av Shishima Hifumi 2911 BR-12244000 Sao Jose Dos Campos SP Brazil;

    Vale Paraiba Univ Res & Dev Inst IPD UNIVAP Av Shishima Hifumi 2911 BR-12244000 Sao Jose Dos Campos SP Brazil;

    Vale Paraiba Univ Res & Dev Inst IPD UNIVAP Av Shishima Hifumi 2911 BR-12244000 Sao Jose Dos Campos SP Brazil;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Photosensitizer; Vaterite submicron particles; Calcium carbonate; Photodynamic therapy;

    机译：光敏剂;Vaterite亚微米粒子;碳酸钙;光动力疗法;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号