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首页> 外文期刊>Photodiagnosis and Photodynamic Therapy >In vitro antimicrobial photodynamic therapy using tetra-cationic porphyrins against multidrug-resistant bacteria isolated from canine otitis
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In vitro antimicrobial photodynamic therapy using tetra-cationic porphyrins against multidrug-resistant bacteria isolated from canine otitis

机译：使用四阳离子卟啉对来自犬耳炎分离的多药抗菌的体外抗微生物动力治疗

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摘要

Antimicrobial-resistant bacteria have been frequently isolated from canine otitis. Photodynamic therapy using porphyrins as photosensitizing molecules is an alternative therapy against microorganisms in localized infections. Therefore, we evaluated the antibacterial activity of two tetra-cationic porphyrins (H2TMeP and ZnTMeP) against gram-positive and -negative bacteria isolated from canine otitis, as well as its putative action mechanism. For this, two gram-positive and two gram-negative bacteria frequently detected in cases of canine otitis (coagulase-positive and -negative staphylococci [CPS and CNS], Pseudomonas aeruginosa, and Proteus mirabilis) were used in antibacterial activity assays. Each porphyrin at a non-cytotoxic dose was incubated with a fixed concentration of each bacterium and exposed to white-light irradiation for 0, 30, 60, and 90 min. Clinical isolates of CPS and CNS were photo-inactivated after 30 min of white-light exposure by both porphyrins (p 0.05). Gram-negative bacteria were also photo-inactivated after 30 min (p 0.05), reaching complete inactivation after 60 and 90 min of white-light irradiation by H2TMeP and ZnTMeP, respectively. Antibacterial assays using standard bacterial strains (ATCCs) demonstrated similar results to those obtained with clinical isolates, except for P. aeruginosa, which was completely inactivated by ZnTMeP at 60 min, and the absence of a significant reduction in P. mirabilis concentration when irradiated for 30 min. Similar assays were conducted using reactive oxygen species scavengers showing that the putative mechanism for bacterial inactivation is through the production of singlet oxygen species. These results indicated that H2TMeP and ZnTMeP tetra-cationic porphyrins were effective against bacteria isolated from canine otitis.
机译：抗菌细菌经常从犬耳炎中分离出来。使用卟啉作为光敏分子的光动力疗法是对局部感染中微生物的替代疗法。因此，我们评估了两种四阳离子卟啉（H2TMEP和ZnTMEP）的抗菌活性，抗犬耳炎分离的革兰氏阳性和阴性细菌，以及其推定的作用机制。为此，在犬耳炎（凝壳酶阳性和阴茎葡萄球菌[CPS和CNS和CNS]，假麦芽糖碱和蛋白质Mirabilis）中经常检测到两种革兰氏阳性和两种革兰氏阴性细菌，用于抗菌活性测定。将非细胞毒剂量的每个卟啉与每个细菌的固定浓度一起孵育，并暴露于0,30,60和90分钟的白光照射。 CPS和CNS的临床分离株在卟啉30分钟后30分钟后的光灭活（P <0.05）。在30分钟后，革兰阴性细菌也是光灭活（P <0.05），分别在H2TMEP和ZnTMEP的60和90分钟后达到完全失活。使用标准细菌菌株（ATCC）的抗菌测定证明了与临床分离株获得的结果相似，除了铜绿假单胞菌，其在60分钟内通过ZnTMEP完全灭活，并且在照射时没有显着降低P. mirabilis浓度。 30分钟。使用反应性氧物种清除剂进行了类似的测定，显示细菌灭活的推定机制是通过生产单次氧物质的产生。这些结果表明，H2TMEP和ZnTMEP四阳离子卟啉针对从犬炎中分离的细菌有效。

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