Molecular authentication of ginseng cultivars by comparison of internal transcribed spacer and 5.8S rDNA sequences

Ok Tae Kim; Kyong Hwan Bang; Dong Su In; Jei Wan Lee; Young Chang Kim; Yoo Soo Shin; Dong Yun Hyun; Sung Sik Lee; Seon Woo Cha; Nak Sul Seong

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Molecular authentication of ginseng cultivars by comparison of internal transcribed spacer and 5.8S rDNA sequences

机译：通过比较内部转录间隔区和5.8S rDNA序列对人参品种进行分子鉴定

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摘要

Molecular authentication among three Panax species and within cultivars and accessions of P. ginseng was investigated using the DNA sequence in the ribosomal ITS1–5.8S–ITS2 region. Four single-nucleotide polymorphisms were identified between P. ginseng and other Panax species. In the electrophoresis profile, obtained after digestion with the enzyme TaqI, three fingerprinting patterns were obtained from cultivars and accessions of Panax species. Consequently, this authentication procedure based upon the restriction fragment length polymorphism in the ribosomal ITS1–5.8S–ITS2 region can now be utilized to differentiate these Panax species as well as major Korean cultivars such as Gopoong and Kumpoong from other cultivars and accessions in Panax species at the DNA level.

机译：利用核糖体ITS1–5.8S–ITS2区域的DNA序列，研究了三种人参之间以及人参体育品种和种质内的分子鉴定。在人参和其他人参物种之间鉴定出四个单核苷酸多态性。在电泳图谱中，用TaqI酶消化后，从人参和栽培品种中获得了三种指纹图谱。因此，基于核糖体ITS1–5.8S–ITS2区域中限制性片段长度多态性的鉴定程序，现在可用于区分这些人参物种以及韩国主要品种，如高浦和甘榜与其他品种和人参在DNA水平上。

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来源
《Plant Biotechnology Reports》 |2007年第3期|163-167|共5页
作者
Ok Tae Kim; Kyong Hwan Bang; Dong Su In; Jei Wan Lee; Young Chang Kim; Yoo Soo Shin; Dong Yun Hyun; Sung Sik Lee; Seon Woo Cha; Nak Sul Seong;
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作者单位

Division of Ginseng and Medicinal Herbs National Institute of Crop Sciences RDA Suwon 441-857 South Korea;

Division of Ginseng and Medicinal Herbs National Institute of Crop Sciences RDA Suwon 441-857 South Korea;

Biotechnology Institute Nongwoo Bio Co. Ltd. Yeoju 469-885 South Korea;

Division of Ginseng and Medicinal Herbs National Institute of Crop Sciences RDA Suwon 441-857 South Korea;

Division of Ginseng and Medicinal Herbs National Institute of Crop Sciences RDA Suwon 441-857 South Korea;

Division of Ginseng and Medicinal Herbs National Institute of Crop Sciences RDA Suwon 441-857 South Korea;

Division of Ginseng and Medicinal Herbs National Institute of Crop Sciences RDA Suwon 441-857 South Korea;

KTampampG Central Research Institute Suwon 441-480 South Korea;

Division of Ginseng and Medicinal Herbs National Institute of Crop Sciences RDA Suwon 441-857 South Korea;

Division of Ginseng and Medicinal Herbs National Institute of Crop Sciences RDA Suwon 441-857 South Korea;

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原文格式 PDF
正文语种 eng
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关键词
Panax species; Molecular authentication; ITS; 5.8S rDNA; Restriction fragment length polymorphism;

机译：人参物种;分子鉴定;ITS;5.8S rDNA;限制性片段长度多态性;

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Molecular authentication of ginseng cultivars by comparison of internal transcribed spacer and 5.8S rDNA sequences

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