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首页> 外文期刊>Plant Cell Reports >Quality evaluation of snow lotus (Saussurea): quantitative chemical analysis and antioxidant activity assessment
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Quality evaluation of snow lotus (Saussurea): quantitative chemical analysis and antioxidant activity assessment

机译：雪莲（Saussurea）的质量评估：定量化学分析和抗氧化活性评估

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Snow lotus is commonly used as a medicinal plant and has great pharmacological value. To protect these endangered plants, in vitro propagation and cell cultures have been established in order to meet the growing market demand. The phenolic composition, antioxidant activities, total phenolic content (TPC) and total flavonoid content (TFC) from three most commonly used species, in vitro propagated lines and the cell cultures were investigated to qualify their pharmacological value. Quantitative analysis showed that the phenolics varied greatly among different species and the same species at different habitats. From this it can be inferred that the phenolics were influenced by genetic background and the environmental conditions. Significant correlations were observed between the antioxidant activity and several phenolics/TPC/TFC, suggesting that the phenolics are a major contributor of the antioxidant activity and are important for quality evaluation of snow lotus. Based on the abundance of phenolics, TPC, TFC and antioxidant activity, the order of the quality for wild species would be Saussurea involucrata > Saussurea medusa > Saussurea gossypiphora. For S. medusa, its quality judged by origin would be Shigatse > Lhasa > Nagqu. For in vitro propagated plants, the matured plants could be a reliable substitute for wild plants, and the dynamics of phenolics is critical for quality control of this monocarpic species. We provide the first report of quality comparison between the wild plants and the cell cultures. The advantages of developing cell cultures as alternatives for plants collected from the wild are discussed.
机译：雪莲被普遍用作药用植物，具有很大的药理价值。为了保护这些濒临灭绝的植物，已经建立了体外繁殖和细胞培养，以满足日益增长的市场需求。研究了三种最常用的物种，体外繁殖系和细胞培养物中的酚类成分，抗氧化剂活性，总酚类含量（TPC）和总类黄酮含量（TFC），以验证其药理价值。定量分析表明，不同生境中同一物种间的酚类差异很大。从中可以推断出酚类化合物受遗传背景和环境条件的影响。观察到抗氧化剂活性与几种酚类/ TPC / TFC之间存在显着相关性，这表明酚类是抗氧化剂活性的主要贡献者，对雪莲的质量评估也很重要。根据酚类，TPC，TFC和抗氧化剂活性的丰富程度，野生物种的质量顺序为：雪莲>美雪莲>棉雪莲。对于水母链球菌，按产地判断的质量为日喀则>拉萨>那曲。对于体外繁殖的植物，成熟的植物可能是野生植物的可靠替代品，而酚类化合物的动力学对于控制这种单生种的质量至关重要。我们提供了野生植物和细胞培养物之间质量比较的第一份报告。讨论了开发细胞培养作为从野外采集的植物的替代品的优势。

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